Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C7S15_RS16985 Genome accession   NZ_QXCU01000001
Coordinates   3584922..3586427 (+) Length   501 a.a.
NCBI ID   WP_318827195.1    Uniprot ID   -
Organism   Burkholderia cepacia strain 10223     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3579922..3591427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7S15_RS16960 (C7S15_3732) kdsD 3581154..3582137 (+) 984 WP_021162595.1 arabinose 5-phosphate isomerase KdsD -
  C7S15_RS16965 (C7S15_3733) - 3582137..3582673 (+) 537 WP_318827194.1 HAD family hydrolase -
  C7S15_RS16970 (C7S15_3734) lptC 3582677..3583291 (+) 615 WP_021162597.1 LPS export ABC transporter periplasmic protein LptC -
  C7S15_RS16975 (C7S15_3735) lptA 3583312..3583977 (+) 666 WP_021162598.1 lipopolysaccharide transport periplasmic protein LptA -
  C7S15_RS16980 (C7S15_3736) lptB 3583974..3584750 (+) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  C7S15_RS16985 (C7S15_3737) rpoN/rpoN1 3584922..3586427 (+) 1506 WP_318827195.1 RNA polymerase factor sigma-54 Machinery gene
  C7S15_RS16990 (C7S15_3738) hpf 3586586..3586945 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  C7S15_RS16995 (C7S15_3739) - 3587114..3587653 (+) 540 WP_034195946.1 PTS sugar transporter subunit IIA -
  C7S15_RS17000 (C7S15_3740) hprK 3587785..3588753 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  C7S15_RS17005 (C7S15_3741) rapZ 3588829..3589737 (+) 909 WP_021162602.1 RNase adapter RapZ -
  C7S15_RS17010 (C7S15_3742) - 3589765..3590400 (+) 636 WP_021162603.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54859.96 Da        Isoelectric Point: 4.8767

>NTDB_id=1171284 C7S15_RS16985 WP_318827195.1 3584922..3586427(+) (rpoN/rpoN1) [Burkholderia cepacia strain 10223]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSERAERDEPAGADEYDGYASGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1171284 C7S15_RS16985 WP_318827195.1 3584922..3586427(+) (rpoN/rpoN1) [Burkholderia cepacia strain 10223]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGATGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCCCAGAACACCGAGCCCGCTGCG
ACAAACAGTAGCAGTTCGTCGAGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTTCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.047

100

0.689

Multiple sequence alignment