Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACOFKY_RS19435 Genome accession   NZ_OZ241355
Coordinates   4163607..4164410 (+) Length   267 a.a.
NCBI ID   WP_336953555.1    Uniprot ID   -
Organism   MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4158607..4169410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKY_RS19410 - 4159255..4160541 (+) 1287 WP_419819971.1 glycosyltransferase family 4 protein -
  ACOFKY_RS19415 - 4160573..4161124 (+) 552 Protein_3796 phosphatase PAP2 family protein -
  ACOFKY_RS19420 iscX 4161343..4161543 (+) 201 WP_004725877.1 Fe-S cluster assembly protein IscX -
  ACOFKY_RS19425 ndk 4161687..4162118 (+) 432 WP_004725878.1 nucleoside-diphosphate kinase -
  ACOFKY_RS19430 rlmN 4162297..4163541 (+) 1245 WP_419327666.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACOFKY_RS19435 pilF 4163607..4164410 (+) 804 WP_336953555.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACOFKY_RS19440 - 4164401..4165189 (+) 789 WP_419358835.1 helix-turn-helix domain-containing protein -
  ACOFKY_RS19445 ispG 4165211..4166326 (+) 1116 WP_004725885.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACOFKY_RS19450 hisS 4166323..4167618 (+) 1296 WP_419819972.1 histidine--tRNA ligase -
  ACOFKY_RS19455 - 4167649..4168350 (+) 702 WP_336953558.1 YfgM family protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30016.18 Da        Isoelectric Point: 9.2123

>NTDB_id=1170804 ACOFKY_RS19435 WP_336953555.1 4163607..4164410(+) (pilF) [MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71]
MYQKAVVILAIGLSALVVQGCQTTNSDTIIKKDPEKAVKVRTQLAAEYIRTGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISLEKADGYFKRAISSDSKNAQARNNYGTYLFVTERYNDAIEQLSIAGTTLGYDQRYKALENLGRVYLKVGD
VANAEKAFKQALQVNRDSIISLVELSEIFYLQQKSVQASQLYEQYVRLVGQDKQGARALWIGLRIARANNDQMGMQVLVN
QLRALFPDSKEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=1170804 ACOFKY_RS19435 WP_336953555.1 4163607..4164410(+) (pilF) [MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71]
ATGTATCAAAAAGCAGTTGTAATATTGGCAATTGGTTTATCTGCATTGGTCGTACAGGGTTGTCAAACGACAAATTCAGA
CACAATAATAAAAAAAGATCCTGAAAAAGCCGTTAAAGTACGTACTCAGCTTGCTGCTGAATATATCCGTACAGGTGATT
TAGATGCTGCAAAACGTGCATTAGATCAAGCTTTAGAAACTGATTCACGTGATTCAGCGGCAAATATGATGATGGGTGTC
TTACTGCAACAAGAAGGAAGTAAGATTAGTCTGGAAAAAGCGGATGGCTATTTTAAGCGTGCGATTTCCAGTGACTCAAA
AAATGCACAAGCTCGTAATAATTATGGAACATATTTATTTGTTACTGAGCGCTATAATGATGCGATAGAACAGTTGAGTA
TAGCTGGTACTACGCTTGGTTATGACCAACGTTACAAGGCTTTAGAAAATCTAGGTCGAGTTTATTTAAAAGTAGGTGAT
GTGGCAAATGCTGAAAAAGCATTCAAACAAGCTTTACAAGTCAATCGCGATTCGATTATTTCTCTGGTAGAGTTATCAGA
GATTTTCTATTTGCAACAAAAAAGTGTACAAGCAAGTCAACTCTATGAGCAATATGTTCGTTTAGTAGGTCAGGACAAAC
AAGGTGCTAGAGCACTTTGGATTGGTTTACGTATTGCACGTGCCAATAATGATCAGATGGGAATGCAAGTGTTGGTGAAC
CAACTACGTGCATTATTCCCGGACAGTAAAGAATATCAACGTTATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(40-69)

Tetratricopeptide repeat (TPR_2)

(146-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

75.094

99.251

0.745
  pilF Acinetobacter baylyi ADP1

68.235

95.506

0.652

Multiple sequence alignment