Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   AW03_RS12370 Genome accession   NZ_CP007173
Coordinates   2411292..2412059 (-) Length   255 a.a.
NCBI ID   WP_015384118.1    Uniprot ID   -
Organism   Bacillus subtilis HJ5     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2406292..2417059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW03_RS12350 (AW03_023800) ppsR 2406466..2407278 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  AW03_RS12355 (AW03_023810) ccpN 2407304..2407942 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  AW03_RS12360 (AW03_023820) glyS 2408075..2410114 (-) 2040 WP_015384117.1 glycine--tRNA ligase subunit beta -
  AW03_RS12365 (AW03_023830) glyQ 2410107..2410994 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  AW03_RS12370 (AW03_023840) recO 2411292..2412059 (-) 768 WP_015384118.1 DNA repair protein RecO Machinery gene
  AW03_RS21230 (AW03_023850) yqzL 2412096..2412239 (-) 144 WP_003230053.1 YqzL family protein -
  AW03_RS12380 (AW03_023860) era 2412387..2413292 (-) 906 WP_003230051.1 GTPase Era -
  AW03_RS12385 (AW03_023870) - 2413273..2413683 (-) 411 WP_003230049.1 cytidine deaminase -
  AW03_RS12390 (AW03_023880) unpK 2413802..2414173 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  AW03_RS12395 (AW03_023890) ybeY 2414154..2414627 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  AW03_RS12400 (AW03_023900) pgpH 2414628..2416763 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29332.01 Da        Isoelectric Point: 8.3080

>NTDB_id=117055 AW03_RS12370 WP_015384118.1 2411292..2412059(-) (recO) [Bacillus subtilis HJ5]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKSNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=117055 AW03_RS12370 WP_015384118.1 2411292..2412059(-) (recO) [Bacillus subtilis HJ5]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTATTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGTC
AAATCCTTATTTATTTGAATTCATACTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment