Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACMFWW_RS01655 Genome accession   NZ_OZ221290
Coordinates   365484..366992 (-) Length   502 a.a.
NCBI ID   WP_017772653.1    Uniprot ID   -
Organism   Paraburkholderia kururiensis isolate ABIP2054     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 360484..371992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMFWW_RS01630 (PK2054_A0354) - 361375..362010 (-) 636 WP_199541742.1 LON peptidase substrate-binding domain-containing protein -
  ACMFWW_RS01635 (PK2054_A0355) rapZ 362078..362971 (-) 894 WP_017772649.1 RNase adapter RapZ -
  ACMFWW_RS01640 (PK2054_A0356) hprK 363038..364006 (-) 969 WP_017772650.1 HPr(Ser) kinase/phosphatase -
  ACMFWW_RS01645 (PK2054_A0357) ptsN 364159..364674 (-) 516 WP_017772651.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACMFWW_RS01650 (PK2054_A0358) hpf 364953..365312 (-) 360 WP_017772652.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACMFWW_RS01655 (PK2054_A0359) rpoN/rpoN1 365484..366992 (-) 1509 WP_017772653.1 RNA polymerase factor sigma-54 Machinery gene
  ACMFWW_RS01660 (PK2054_A0360) lptB 367283..368065 (-) 783 WP_017772654.1 LPS export ABC transporter ATP-binding protein -
  ACMFWW_RS01665 (PK2054_A0361) lptA 368062..368739 (-) 678 WP_017772655.1 lipopolysaccharide transport periplasmic protein LptA -
  ACMFWW_RS01670 (PK2054_A0362) lptC 368772..369371 (-) 600 WP_413212977.1 LPS export ABC transporter periplasmic protein LptC -
  ACMFWW_RS01675 (PK2054_A0363) - 369368..369916 (-) 549 WP_017772657.1 KdsC family phosphatase -
  ACMFWW_RS01680 (PK2054_A0364) kdsD 369916..370899 (-) 984 WP_413212978.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54950.07 Da        Isoelectric Point: 5.0002

>NTDB_id=1170524 ACMFWW_RS01655 WP_017772653.1 365484..366992(-) (rpoN/rpoN1) [Paraburkholderia kururiensis isolate ABIP2054]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENEDDWIASPLRVAADGSVIAQTPTPSAPEPMS
AAPSSSSSAESSESGEPASVDEYNGLGSDANGDASQWNLDDYGRSGNASDDDDLPPLQIHESTTSLRDYLTAQLRVTQAS
PRDRALITFLIESLDDEGYLTATCEEILADLPVELEVDADELNAALALLQSFDPPGVGARSASECLKLQLLRLDPSPTRT
LALEIVAHHLELLAARDFTRLRKHLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDVIVKKTAQGWHAELNPEVV
PKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFVHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=1170524 ACMFWW_RS01655 WP_017772653.1 365484..366992(-) (rpoN/rpoN1) [Paraburkholderia kururiensis isolate ABIP2054]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCATCTTGCGCTGACCCCCCAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCTACGCTCGAATTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCCCTGCTCGAAAACGAAGACGACTGGA
TCGCCAGCCCGTTGCGGGTGGCGGCAGACGGTTCCGTCATCGCGCAAACGCCCACGCCTTCGGCGCCCGAGCCGATGTCC
GCTGCACCCTCGTCGTCTTCCTCGGCAGAGAGTTCGGAAAGCGGCGAACCCGCCAGCGTCGACGAATACAACGGCCTCGG
CTCCGATGCCAACGGCGATGCGTCGCAGTGGAACCTCGACGACTATGGCCGCTCCGGCAACGCGTCCGACGACGACGACC
TTCCGCCGCTGCAGATTCACGAATCCACCACGTCGCTGCGCGACTACCTGACGGCACAGTTGCGCGTCACGCAGGCAAGC
CCGCGTGACCGCGCGCTCATCACGTTCCTCATCGAATCGCTCGACGACGAAGGCTACCTCACGGCAACGTGTGAAGAAAT
ACTGGCCGACTTGCCGGTCGAGCTCGAGGTGGACGCCGACGAGTTGAATGCGGCACTCGCATTGCTGCAAAGCTTCGACC
CGCCTGGCGTCGGCGCGCGTTCGGCATCGGAATGCCTGAAGCTGCAGTTGCTTCGGCTCGATCCGTCGCCTACTCGCACG
CTCGCGCTCGAAATCGTCGCGCACCATCTGGAGTTGCTTGCCGCACGAGACTTCACTCGTCTGCGCAAACATCTGAAGGC
AAGCGACGACGAACTGCGCGACGCCCATGCGCTGATCCGCTCGCTCGAACCGTTCCCGGGTGCGGCCTACGGCAAGGCCG
AAGCCGACTACGTCGTGCCCGACGTGATCGTGAAGAAGACCGCACAAGGATGGCACGCCGAACTGAATCCCGAGGTCGTG
CCGAAGCTGCGCATCAATCATCTGTACGCGAACATCCTGCGCAACAACCGCGGCGATCCGGGCAGCGGCTCGTTGCGTCA
GCAACTGCAGGAAGCCCGCTGGTTGATCAAAAATATTCAGCAGCGCTTCGAAACAATCCTGCGTGTTGCGCAGGCCATTG
TGGAGCGTCAGAAGAACTTTTTTGTGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCTTACACGAGTCAACTGTCTCGCGTGTGACAACCGGCAAGTACATGCTCACCCCGTTTGGGACGCTTGAATTTAAGTA
CTTCTTCGGATCGCATGTTTCGACGGATACGGGAGGCGCGGCCTCATCCACGGCCATACGCGCGCTCATCAAGCAACTGA
TAGGAGCAGAGAACCCGAAATCCCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTCGTGGTGGCG
CGCCGCACGGTGGCGAAGTATCGCGAGGCGCTCAAGATTCCAGCGGTCAACCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-329)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.327

100

0.683

Multiple sequence alignment