Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   ACLV7A_RS01495 Genome accession   NZ_OZ217344
Coordinates   281116..281940 (+) Length   274 a.a.
NCBI ID   WP_411863402.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis E22     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 276116..286940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7A_RS01460 (SMIE22_02800) asnA 276206..277198 (+) 993 WP_000748005.1 aspartate--ammonia ligase -
  ACLV7A_RS01465 (SMIE22_02810) rsmD 277263..277802 (+) 540 WP_000706954.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACLV7A_RS01470 (SMIE22_02820) coaD 277792..278280 (+) 489 WP_261060069.1 pantetheine-phosphate adenylyltransferase -
  ACLV7A_RS01475 (SMIE22_02830) sepM 278264..279301 (+) 1038 WP_411863400.1 SepM family pheromone-processing serine protease Regulator
  ACLV7A_RS01480 (SMIE22_02840) - 279359..279589 (+) 231 WP_078135418.1 DUF1146 family protein -
  ACLV7A_RS01485 (SMIE22_02850) murA 279613..280896 (+) 1284 WP_411863401.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  ACLV7A_RS01490 (SMIE22_02860) - 280889..281077 (+) 189 WP_162499100.1 DNA-directed RNA polymerase subunit beta -
  ACLV7A_RS01495 (SMIE22_02870) endA 281116..281940 (+) 825 WP_411863402.1 DNA/RNA non-specific endonuclease Machinery gene
  ACLV7A_RS01500 (SMIE22_02880) - 282095..283426 (+) 1332 WP_411863403.1 hemolysin family protein -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29773.34 Da        Isoelectric Point: 9.8803

>NTDB_id=1170265 ACLV7A_RS01495 WP_411863402.1 281116..281940(+) (endA) [Streptococcus mitis isolate S. mitis E22]
MNKKTRQTLIGLLVLLLLSAGSYYIKQTELGNQAAKTKMSQKSQSPDAPGQALAESVLTDAVKSQVKGSLEWNGSGAFIV
NGNKTNLNAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGTYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESLVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=1170265 ACLV7A_RS01495 WP_411863402.1 281116..281940(+) (endA) [Streptococcus mitis isolate S. mitis E22]
ATGAACAAAAAAACAAGACAGACACTGATTGGTTTGCTAGTGTTATTGCTTTTATCTGCGGGGAGCTATTATATTAAGCA
GACTGAACTAGGAAATCAGGCTGCTAAGACAAAAATGAGTCAGAAAAGTCAATCACCAGACGCGCCTGGTCAAGCATTGG
CAGAAAGTGTCTTAACAGATGCAGTCAAAAGCCAAGTAAAAGGGAGTCTGGAGTGGAACGGATCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAACCTAAATGCCAAGGTTTCAAGTAAGCCCTATGCTGATAATAAAACAAAAACGGTGGGCAAGGA
GACCGTACCAACCGTGGCTAATGCCCTCTTGTCTAAGGCTACTCGCCAGTACAAGAATCGTGAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTTAAGAATCTAAAGGGTACTTATACCCATGCGGTCGATAGAGGTCAC
TTGTTAGGCTATGCCCTAATCGGTGGCTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTCCAAAC
AGCCTGGGCAAATCAGGCGCAAGCTGAAGATTCGACAGGGCAAAACTACTATGAAAGTCTGGTGCGTAAAGCCCTAGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTATTATGCTTCAAACGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCCTCAGACGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAAGGGCTTCAACTGGATTACCGCAC
TGGAGAAGTCACTGTTACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

91.971

100

0.92
  endA Streptococcus pneumoniae D39

91.971

100

0.92
  endA Streptococcus pneumoniae R6

91.971

100

0.92
  endA Streptococcus pneumoniae TIGR4

91.971

100

0.92

Multiple sequence alignment