Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   ACLV7Q_RS01465 Genome accession   NZ_OZ217341
Coordinates   272059..272883 (+) Length   274 a.a.
NCBI ID   WP_218757215.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis C22     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 267059..277883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Q_RS01430 (SMIC22_02710) asnA 267149..268141 (+) 993 WP_000748003.1 aspartate--ammonia ligase -
  ACLV7Q_RS01435 (SMIC22_02720) rsmD 268206..268745 (+) 540 WP_218757219.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACLV7Q_RS01440 (SMIC22_02730) coaD 268735..269223 (+) 489 WP_218757218.1 pantetheine-phosphate adenylyltransferase -
  ACLV7Q_RS01445 (SMIC22_02740) sepM 269207..270244 (+) 1038 WP_218757217.1 SepM family pheromone-processing serine protease Regulator
  ACLV7Q_RS01450 (SMIC22_02750) - 270302..270532 (+) 231 WP_000250395.1 DUF1146 family protein -
  ACLV7Q_RS01455 (SMIC22_02760) murA 270556..271839 (+) 1284 WP_218757216.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  ACLV7Q_RS01460 (SMIC22_02770) - 271826..272020 (+) 195 WP_050236587.1 DNA-directed RNA polymerase subunit beta -
  ACLV7Q_RS01465 (SMIC22_02780) endA 272059..272883 (+) 825 WP_218757215.1 DNA/RNA non-specific endonuclease Machinery gene
  ACLV7Q_RS01470 (SMIC22_02790) - 273038..274369 (+) 1332 WP_218757214.1 hemolysin family protein -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29856.41 Da        Isoelectric Point: 9.8622

>NTDB_id=1170015 ACLV7Q_RS01465 WP_218757215.1 272059..272883(+) (endA) [Streptococcus mitis isolate S. mitis C22]
MNKKTRQTLIGLLVLLLLSAGSYYIKQNELGNQAAKTKISQKSQSPDAPGQELAESVLTDAVKSQIKGSLEWNGAGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESKVRKALDKNKRVRYRVTLYYTSNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=1170015 ACLV7Q_RS01465 WP_218757215.1 272059..272883(+) (endA) [Streptococcus mitis isolate S. mitis C22]
ATGAACAAAAAAACAAGACAGACACTGATTGGCTTGCTAGTGTTATTGCTCTTATCTGCGGGGAGCTATTATATTAAGCA
GAATGAACTAGGCAATCAGGCTGCTAAGACAAAAATAAGTCAGAAAAGTCAATCACCAGACGCGCCTGGTCAAGAATTGG
CGGAAAGTGTCTTAACAGATGCAGTCAAAAGCCAAATAAAAGGGAGTCTGGAGTGGAACGGGGCTGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCTAAGGTTTCAAGTAAGCCCTACGCTGATAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTGGCTAATGCCCTCTTGTCTAAGGCTACTCGCCAGTACAAGAATCGTGAAGAAACTGGAAACGGTT
CGACTTCATGGACTCCTCCAGGTTGGCATCAGGTTAAGAATCTAAAGGGCTCTTATACCCATGCCGTTGATAGAGGGCAC
TTGTTAGGCTATGCCTTAATCGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCGCAGGCCGAGGATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAGGCCTTGGATA
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACACTTCGAACGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGATGGAGAATTGGAATTTAATGTCCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTATCGAAC
TGGAGAAGTCACTGTTACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(76-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

91.971

100

0.92
  endA Streptococcus pneumoniae D39

91.971

100

0.92
  endA Streptococcus pneumoniae R6

91.971

100

0.92
  endA Streptococcus pneumoniae TIGR4

91.971

100

0.92

Multiple sequence alignment