Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG47_RS03985 Genome accession   NZ_CP010169
Coordinates   744849..745526 (+) Length   225 a.a.
NCBI ID   WP_073464236.1    Uniprot ID   -
Organism   Escherichia coli strain H5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 739849..750526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG47_RS03950 (RG47_03770) gspG 740329..740766 (+) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -
  RG47_RS03955 (RG47_03775) gspH 740774..741283 (+) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  RG47_RS03960 (RG47_03780) gspI 741280..741657 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RG47_RS03965 (RG47_03785) gspJ 741650..742237 (+) 588 WP_063090498.1 type II secretion system minor pseudopilin GspJ -
  RG47_RS03970 (RG47_03790) gspK 742230..743213 (+) 984 WP_073464238.1 type II secretion system minor pseudopilin GspK -
  RG47_RS03975 (RG47_03795) gspL 743228..744391 (+) 1164 WP_001115230.1 type II secretion system protein GspL -
  RG47_RS03980 (RG47_03800) gspM 744388..744849 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  RG47_RS03985 (RG47_03805) pilD 744849..745526 (+) 678 WP_073464236.1 prepilin peptidase GspO Machinery gene
  RG47_RS03990 (RG47_03810) bfr 745555..746031 (-) 477 WP_000675504.1 bacterioferritin -
  RG47_RS03995 (RG47_03815) bfd 746103..746297 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RG47_RS04000 (RG47_03820) chiA 746466..749159 (-) 2694 WP_073464234.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24912.81 Da        Isoelectric Point: 7.9564

>NTDB_id=116807 RG47_RS03985 WP_073464236.1 744849..745526(+) (pilD) [Escherichia coli strain H5]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIELHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=116807 RG47_RS03985 WP_073464236.1 744849..745526(+) (pilD) [Escherichia coli strain H5]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGAGTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382

Multiple sequence alignment