Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEN42_RS14145 Genome accession   NZ_OZ180035
Coordinates   3024665..3026182 (+) Length   505 a.a.
NCBI ID   WP_187607436.1    Uniprot ID   -
Organism   Caballeronia sp. S22 isolate ena-SAMPLE-TAB-09-04-2024-15:28:32:345-2572     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3019665..3031182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN42_RS14120 (CABS22_A2987) kdsD 3020885..3021868 (+) 984 WP_238285248.1 arabinose 5-phosphate isomerase KdsD -
  ACEN42_RS14125 (CABS22_A2988) - 3021868..3022407 (+) 540 WP_371880256.1 KdsC family phosphatase -
  ACEN42_RS14130 (CABS22_A2989) lptC 3022413..3023015 (+) 603 WP_187607438.1 LPS export ABC transporter periplasmic protein LptC -
  ACEN42_RS14135 (CABS22_A2990) lptA 3023046..3023732 (+) 687 WP_187607437.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEN42_RS14140 (CABS22_A2991) lptB 3023744..3024511 (+) 768 WP_187607615.1 LPS export ABC transporter ATP-binding protein -
  ACEN42_RS14145 (CABS22_A2992) rpoN/rpoN1 3024665..3026182 (+) 1518 WP_187607436.1 RNA polymerase factor sigma-54 Machinery gene
  ACEN42_RS14150 (CABS22_A2993) hpf 3026367..3026726 (+) 360 WP_014190335.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEN42_RS14155 (CABS22_A2994) - 3026838..3027104 (+) 267 WP_187607435.1 hypothetical protein -
  ACEN42_RS14160 (CABS22_A2995) ptsN 3027137..3027592 (+) 456 WP_187607434.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEN42_RS14165 (CABS22_A2996) hprK 3027778..3028746 (+) 969 WP_008351692.1 HPr(Ser) kinase/phosphatase -
  ACEN42_RS14170 (CABS22_A2997) rapZ 3028822..3029694 (+) 873 WP_187607433.1 RNase adapter RapZ -
  ACEN42_RS14175 (CABS22_A2998) - 3029754..3030386 (+) 633 WP_187607432.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55545.51 Da        Isoelectric Point: 4.8712

>NTDB_id=1167954 ACEN42_RS14145 WP_187607436.1 3024665..3026182(+) (rpoN/rpoN1) [Caballeronia sp. S22 isolate ena-SAMPLE-TAB-09-04-2024-15:28:32:345-2572]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDDWITSPLRVAADGSLIAAPNTPSTPPDSM
MSNGSSSSSSSSERSENSEPQGVDEYNGMGSDSAQDSSSWNLEDYGRSNNASDDDDLPPLQIHESTTTLRDHLTSQLRMT
AANQRDRALVTFLIESLDEDGYLTSSLDEVQTDLPEELEVDLDELSAALALLQSFDPPGVGGRSASECLKLQLLRLDGSP
TRTLALDIVTNHLELLAARDFTRLRKQLKANDDALRDAHVLIRSLEPFPGAAYGKSEADYVVPDILVRKAASGWTAELNP
EIVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=1167954 ACEN42_RS14145 WP_187607436.1 3024665..3026182(+) (rpoN/rpoN1) [Caballeronia sp. S22 isolate ena-SAMPLE-TAB-09-04-2024-15:28:32:345-2572]
ATGAAAGCCAGCCTCCAACTTCGCCTCTCGCAGCATCTTGCGCTGACCCCGCAACTTCAGCAGTCCATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCTCAGAATCCGCTGCTCGAGAATGACGACGACTGGA
TCACGAGCCCGCTGCGCGTCGCCGCGGACGGCTCGCTGATAGCGGCGCCGAACACGCCGAGCACGCCGCCCGATTCGATG
ATGAGCAACGGCTCGTCATCGAGCAGTTCCAGTTCCGAACGTTCGGAAAATAGCGAACCGCAAGGCGTCGACGAATACAA
CGGGATGGGTTCGGACAGCGCTCAGGATTCCAGTTCGTGGAACCTCGAGGACTACGGCCGTTCGAACAACGCGTCCGACG
ACGACGATCTCCCGCCGCTGCAAATCCACGAATCGACCACGACGCTGCGCGACCATCTGACCTCGCAGTTGCGCATGACC
GCGGCGAATCAGCGCGATCGTGCGCTCGTCACGTTTCTGATCGAATCGCTGGACGAGGACGGGTATCTGACATCATCGCT
CGATGAAGTTCAGACCGATCTTCCCGAAGAGCTCGAAGTCGATCTCGACGAGTTGAGCGCGGCGCTCGCCCTGCTGCAAA
GCTTCGATCCGCCCGGCGTGGGCGGACGTTCGGCATCGGAATGCCTGAAGCTGCAGTTGCTGCGGCTCGACGGCTCGCCC
ACGCGCACGCTCGCGCTCGATATCGTCACGAATCATCTGGAACTGCTCGCGGCGCGCGACTTCACGCGTCTGCGCAAGCA
ACTGAAGGCCAACGACGACGCGCTGCGCGACGCGCATGTGCTGATCAGATCGCTGGAGCCTTTCCCCGGCGCCGCGTACG
GCAAGAGCGAAGCGGACTATGTCGTGCCGGATATTCTCGTGCGAAAGGCGGCGAGCGGATGGACGGCGGAGCTGAATCCG
GAGATCGTGCCGAAGCTGCGCATCAACCATCTGTACGCGAACATCCTGCGCAACAATCGCGGCGATCCGGGCAGCGGCTC
GCTGCGTCAGCAACTCCAGGAAGCGCGCTGGCTCATCAAGAATATTCAGCAGAGGTTTGAGACGATTCTGCGTGTCGCGC
AGGCGATCGTGGAGCGTCAGAAGAACTTTTTTGCACATGGCGAAATCGCCATGCGGCCCTTGGTTTTGCGGGAGATTGCT
GATACGCTGGGATTACACGAATCGACGGTTTCTCGTGTGACCACAGGCAAGTACATGCTCACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTTGGATCGCACGTTTCGACCGACACAGGAGGCGCGGCATCGTCAACGGCGATCCGGGCCCTCATCA
AGCAACTGATAGGAGCGGAAGACCCCAAAACTCCTCTTTCAGACAGCCGCATCGCCGAACTGCTGGCGGAACAAGGATTC
GTGGTCGCGCGGCGCACCGTCGCCAAGTACCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTATGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.139

100

0.661

Multiple sequence alignment