Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   AW02_RS08375 Genome accession   NZ_CP007165
Coordinates   1699169..1700068 (+) Length   299 a.a.
NCBI ID   WP_015388238.1    Uniprot ID   A0A6A8LH26
Organism   Bacillus velezensis NJN-6     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1694169..1705068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW02_RS08355 (AW02_016330) - 1694860..1696563 (+) 1704 WP_024085298.1 hypothetical protein -
  AW02_RS08360 (AW02_016340) - 1696560..1696841 (+) 282 WP_003154284.1 FlhB-like flagellar biosynthesis protein -
  AW02_RS08365 (AW02_016350) sucC 1697016..1698173 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  AW02_RS08370 (AW02_016360) sucD 1698202..1699104 (+) 903 WP_003154281.1 succinate--CoA ligase subunit alpha -
  AW02_RS08375 (AW02_016370) dprA 1699169..1700068 (+) 900 WP_015388238.1 DNA-processing protein DprA Machinery gene
  AW02_RS08380 (AW02_016380) topA 1700250..1702325 (+) 2076 WP_003154274.1 type I DNA topoisomerase -
  AW02_RS08385 (AW02_016390) trmFO 1702390..1703697 (+) 1308 WP_003154273.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  AW02_RS08390 (AW02_016400) xerC 1703767..1704684 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32820.92 Da        Isoelectric Point: 7.8285

>NTDB_id=116705 AW02_RS08375 WP_015388238.1 1699169..1700068(+) (dprA) [Bacillus velezensis NJN-6]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTVLSEGKTAPEAIFREIERKDPELDEVLSDYRREGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTPLLEKGRKIGIVGTRKPTEDGIKAAGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=116705 AW02_RS08375 WP_015388238.1 1699169..1700068(+) (dprA) [Bacillus velezensis NJN-6]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGATCACTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGGATCCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCGAAGGCATTACTGTCATT
CCGATTTCATCAAGCCGCTACCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGAAACAC
ACCGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGCTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGTTTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCACATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCCCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LH26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.569

100

0.706
  dprA Lactococcus lactis subsp. cremoris KW2

41.288

88.294

0.365

Multiple sequence alignment