Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AW02_RS01725 Genome accession   NZ_CP007165
Coordinates   356069..356506 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis NJN-6     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 351069..361506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW02_RS20820 - 351649..351774 (-) 126 Protein_305 hypothetical protein -
  AW02_RS01695 (AW02_003030) - 351927..353129 (+) 1203 WP_024084872.1 GTP-binding protein -
  AW02_RS01700 (AW02_003040) - 353194..354330 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  AW02_RS01705 (AW02_003050) - 354345..354779 (+) 435 WP_021494203.1 RDD family protein -
  AW02_RS01710 (AW02_003060) - 354896..355171 (+) 276 WP_369719109.1 YckD family protein -
  AW02_RS21100 - 355275..355358 (+) 84 WP_223151977.1 family 1 glycosylhydrolase -
  AW02_RS01715 - 355390..355608 (+) 219 Protein_311 family 1 glycosylhydrolase -
  AW02_RS01720 (AW02_003070) nin/comJ 355649..356047 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  AW02_RS01725 (AW02_003080) nucA/comI 356069..356506 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AW02_RS01730 (AW02_003090) hxlB 356865..357422 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  AW02_RS01735 (AW02_003100) hxlA 357419..358054 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  AW02_RS01740 (AW02_003110) - 358286..358648 (+) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=116681 AW02_RS01725 WP_003156588.1 356069..356506(-) (nucA/comI) [Bacillus velezensis NJN-6]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=116681 AW02_RS01725 WP_003156588.1 356069..356506(-) (nucA/comI) [Bacillus velezensis NJN-6]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment