Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   AB3Y89_RS07485 Genome accession   NZ_OZ061338
Coordinates   1432382..1432894 (-) Length   170 a.a.
NCBI ID   WP_025016386.1    Uniprot ID   -
Organism   Latilactobacillus sakei isolate Latilactobacillus sakei J33     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1427382..1437894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3Y89_RS07455 - 1428308..1428673 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  AB3Y89_RS07465 - 1429216..1429674 (-) 459 WP_011374992.1 hypothetical protein -
  AB3Y89_RS07470 - 1429729..1430925 (-) 1197 WP_011374993.1 acetate/propionate family kinase -
  AB3Y89_RS07475 - 1430947..1431957 (-) 1011 WP_099947695.1 class I SAM-dependent methyltransferase -
  AB3Y89_RS07480 - 1432081..1432419 (-) 339 WP_056947123.1 hypothetical protein -
  AB3Y89_RS07485 comGF 1432382..1432894 (-) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  AB3Y89_RS07490 comGE 1432869..1433174 (-) 306 WP_025016385.1 hypothetical protein Machinery gene
  AB3Y89_RS07495 comGD 1433161..1433613 (-) 453 WP_076632063.1 competence type IV pilus minor pilin ComGD Machinery gene
  AB3Y89_RS07500 comGC 1433585..1433884 (-) 300 WP_076632062.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AB3Y89_RS07505 comGB 1433881..1434888 (-) 1008 WP_076632061.1 type II secretion system F family protein Machinery gene
  AB3Y89_RS07510 comGA 1434881..1435771 (-) 891 WP_367294528.1 competence type IV pilus ATPase ComGA Machinery gene
  AB3Y89_RS07515 - 1435887..1436618 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AB3Y89_RS07520 - 1436709..1437209 (-) 501 WP_099947696.1 VanZ family protein -
  AB3Y89_RS07525 - 1437306..1437680 (+) 375 WP_011375004.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19690.57 Da        Isoelectric Point: 6.6472

>NTDB_id=1166024 AB3Y89_RS07485 WP_025016386.1 1432382..1432894(-) (comGF) [Latilactobacillus sakei isolate Latilactobacillus sakei J33]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLDGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=1166024 AB3Y89_RS07485 WP_025016386.1 1432382..1432894(-) (comGF) [Latilactobacillus sakei isolate Latilactobacillus sakei J33]
TTGCGATTAATTGGACGACCGATTAAACACTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTGACACAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCAGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTCGTTGTCAGCCAATTAGATGGGTATATGCCATTATTAGGTAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACATGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.824

100

0.988

Multiple sequence alignment