Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   AB3227_RS03565 Genome accession   NZ_OZ060996
Coordinates   753818..755629 (+) Length   603 a.a.
NCBI ID   WP_014939610.1    Uniprot ID   J9W275
Organism   Lentilactobacillus buchneri isolate Lentilactobacillus buchneri CIRM-BIA1514     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 748818..760629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3227_RS03545 - 748863..749555 (+) 693 WP_013727493.1 response regulator transcription factor -
  AB3227_RS03550 - 749552..750862 (+) 1311 WP_013727494.1 cell wall metabolism sensor histidine kinase WalK -
  AB3227_RS03555 - 750950..752488 (-) 1539 WP_014939608.1 gamma-glutamylcysteine synthetase -
  AB3227_RS03560 - 752644..753783 (+) 1140 WP_014939609.1 competence protein CoiA -
  AB3227_RS03565 pepF 753818..755629 (+) 1812 WP_014939610.1 oligoendopeptidase F Regulator
  AB3227_RS03570 - 755703..756323 (-) 621 WP_013727498.1 DsbA family protein -
  AB3227_RS03575 - 756538..757191 (+) 654 WP_013727499.1 GTP pyrophosphokinase family protein -
  AB3227_RS03580 - 757169..757981 (+) 813 WP_013727500.1 NAD kinase -
  AB3227_RS03585 - 757974..758864 (+) 891 WP_014939611.1 RluA family pseudouridine synthase -
  AB3227_RS03590 - 758974..759822 (+) 849 WP_014939612.1 methyltransferase domain-containing protein -
  AB3227_RS03595 - 759840..760352 (+) 513 WP_014939613.1 tRNA (cytidine(34)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 69704.80 Da        Isoelectric Point: 4.9207

>NTDB_id=1165491 AB3227_RS03565 WP_014939610.1 753818..755629(+) (pepF) [Lentilactobacillus buchneri isolate Lentilactobacillus buchneri CIRM-BIA1514]
MPKPQELPLRKDVPEELTWDLTTIYPTDQEFETDYDAVQTGAKQFAELAGTIEKSAASLLTATERLMTLSRKLEKVYVYA
QLKNDQDTSEDKYQAMYSRAESLGAQFGAATAWFEPELLKISDDQIKQFYQDEPKLADYRRFFDQTFKRRNHILSNDVEA
VLAGAGDIFSSGEKTFGILDNTDLSFPAVTDENGNQVKLSQGVYGILLESTKQSVRKEAFEKLYQVYRQFQHTLAATLTT
NVKNHNFKANIRHYNNALEAALADNEVPTDVYNNLIKVVNEHLDLLHRYVALRKKILGLDELHMYDMYTSLVGKKSPKYT
FEQSKQIALEALQVMGPDYVSHVKEEFNNRWIDVVENQFKRSGGYSSGTYDTNPYILLNWKDNLDNLYTLIHETGHSMHS
YYSHHNQPYQYGDYSIFVAEIASTTNENILTDYLLNKFKDDDEMKKFILNYYLDGFKGTVFRQTQFAEFEQYIHEQDAKG
QPLTANFMDDYYRNLNQKYYGDAVISDPQIGLEWSRIPHFYYNFYVYQYSTGFAAASTLADGIVNGGQANVDKYLNYLKS
GSSDTPTNVMKKAGVDMTQPEYLEKAFQIFEQRLDEFERLYNK

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=1165491 AB3227_RS03565 WP_014939610.1 753818..755629(+) (pepF) [Lentilactobacillus buchneri isolate Lentilactobacillus buchneri CIRM-BIA1514]
ATGCCAAAACCACAAGAATTACCGCTTAGAAAAGATGTTCCAGAAGAACTGACTTGGGACCTGACGACGATTTATCCAAC
CGATCAGGAATTTGAAACTGACTATGACGCTGTTCAAACCGGTGCCAAACAATTCGCTGAATTAGCCGGAACCATTGAGA
AGAGTGCGGCCTCACTGCTGACAGCAACTGAGCGTTTGATGACCTTAAGTCGAAAGCTTGAAAAAGTCTATGTCTACGCC
CAGTTGAAAAATGACCAGGATACCAGTGAAGACAAGTATCAGGCCATGTATTCCCGTGCGGAATCCTTGGGGGCTCAATT
TGGGGCGGCAACTGCCTGGTTTGAACCGGAACTCCTCAAAATTTCTGACGATCAAATCAAGCAGTTTTATCAGGATGAAC
CCAAATTGGCCGATTATCGACGGTTTTTCGATCAAACCTTTAAACGCCGTAATCATATTTTAAGCAACGATGTTGAAGCC
GTTCTGGCCGGGGCCGGCGATATTTTCTCGTCTGGAGAGAAAACCTTTGGCATTTTGGATAACACTGATCTTTCATTCCC
AGCGGTCACTGATGAGAATGGTAATCAGGTGAAGTTATCTCAAGGTGTTTATGGTATTTTATTGGAATCGACAAAGCAAA
GTGTGCGTAAAGAAGCGTTTGAAAAATTGTATCAAGTCTATCGTCAGTTCCAGCACACGCTGGCGGCAACACTGACCACT
AATGTTAAGAATCACAACTTCAAAGCCAACATCCGCCACTATAACAATGCATTGGAGGCCGCTTTGGCTGATAACGAAGT
GCCGACAGACGTTTATAACAACCTGATCAAAGTGGTCAACGAGCATCTCGATCTGCTGCACAGGTACGTTGCTTTGCGTA
AAAAGATTCTGGGCCTTGATGAACTGCATATGTACGATATGTATACGTCCCTAGTTGGTAAAAAATCACCCAAGTATACG
TTTGAACAATCCAAGCAAATCGCCTTGGAGGCTTTGCAGGTAATGGGGCCGGATTATGTCAGCCACGTCAAAGAGGAGTT
CAACAACCGTTGGATTGACGTTGTTGAAAATCAATTCAAAAGAAGCGGCGGCTATTCCTCCGGGACTTACGATACCAATC
CGTATATTTTACTGAATTGGAAGGACAATTTGGATAATCTCTACACGCTGATCCACGAAACCGGTCATAGTATGCACAGC
TACTATTCCCATCATAACCAGCCTTATCAGTATGGGGATTACTCCATTTTTGTGGCTGAAATTGCGTCCACGACTAACGA
GAATATCTTGACGGATTATCTCTTGAATAAATTCAAAGATGACGATGAAATGAAGAAATTTATTTTAAATTACTATCTGG
ATGGATTTAAGGGAACGGTCTTCCGTCAAACTCAGTTTGCTGAATTTGAACAATACATTCATGAACAGGATGCAAAAGGT
CAACCATTAACCGCCAACTTTATGGATGATTACTATCGCAATTTAAATCAAAAATACTACGGGGATGCCGTGATTTCTGA
CCCTCAGATTGGTCTTGAATGGTCACGGATTCCACATTTCTATTACAACTTCTACGTTTACCAATATTCGACCGGCTTTG
CTGCCGCATCGACATTGGCTGATGGCATTGTGAACGGCGGCCAGGCCAACGTTGATAAATATTTGAATTACCTCAAATCA
GGCAGTTCTGACACGCCAACCAACGTCATGAAAAAAGCCGGCGTTGACATGACGCAACCGGAATACCTTGAGAAGGCCTT
CCAGATTTTTGAGCAGCGTCTGGATGAATTTGAGCGTTTGTACAACAAATAG

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(117-186)

Peptidase family M3 (Peptidase_M3)

(207-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J9W275

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

53.03

98.507

0.522

Multiple sequence alignment