Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACE4RE_RS00760 Genome accession   NZ_OZ059822
Coordinates   157937..159301 (-) Length   454 a.a.
NCBI ID   WP_001085219.1    Uniprot ID   W8U6A9
Organism   Staphylococcus aureus isolate E9_23S01315-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 152937..164301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE4RE_RS00745 cysE 153758..154405 (-) 648 WP_001805237.1 serine O-acetyltransferase -
  ACE4RE_RS00750 gltX 154828..156282 (-) 1455 WP_001283787.1 glutamate--tRNA ligase -
  ACE4RE_RS00755 - 156839..157912 (-) 1074 WP_001028375.1 PIN/TRAM domain-containing protein -
  ACE4RE_RS00760 radA 157937..159301 (-) 1365 WP_001085219.1 DNA repair protein RadA Machinery gene
  ACE4RE_RS00765 - 159786..162242 (-) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  ACE4RE_RS00770 - 162256..163263 (-) 1008 WP_000149503.1 protein arginine kinase -
  ACE4RE_RS00775 - 163253..163819 (-) 567 WP_000882071.1 UvrB/UvrC motif-containing protein -
  ACE4RE_RS00780 - 163838..164299 (-) 462 WP_000551762.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49835.35 Da        Isoelectric Point: 7.0049

>NTDB_id=1165282 ACE4RE_RS00760 WP_001085219.1 157937..159301(-) (radA) [Staphylococcus aureus isolate E9_23S01315-1]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1165282 ACE4RE_RS00760 WP_001085219.1 157937..159301(-) (radA) [Staphylococcus aureus isolate E9_23S01315-1]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGAAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTGAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCAAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTGTTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTACTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCACCAGGTTCTGT
TTCACAAGTTCGTGAAAGCACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATCTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCGTTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U6A9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.674
  radA Streptococcus pneumoniae Rx1

62.054

98.678

0.612
  radA Streptococcus pneumoniae D39

62.054

98.678

0.612
  radA Streptococcus pneumoniae R6

62.054

98.678

0.612
  radA Streptococcus pneumoniae TIGR4

62.054

98.678

0.612
  radA Streptococcus mitis NCTC 12261

62.054

98.678

0.612
  radA Streptococcus mitis SK321

62.054

98.678

0.612

Multiple sequence alignment