Detailed information NTG1165147

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: ACE4RG_RS02385
• Genome accession: NZ_OZ059819
• Coordinates: 488828..490177 (+)
• Length: 449 a.a.
• NCBI ID: WP_000815652.1
• Uniprot ID: Q8VUV4
• Organism: Staphylococcus aureus isolate H2_22S00635-1
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	483221..498592	488828..490177	within	0

Gene organization within MGE regions

Location: 483221..498592

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE4RG_RS02365	-	483221..484711 (-)	1491	WP_000554504.1	RNA-binding domain-containing protein	-
ACE4RG_RS02370	-	485329..486378 (-)	1050	WP_000613248.1	hypothetical protein	-
ACE4RG_RS02375	ssb	486517..486807 (+)	291	WP_001036437.1	cassette chromosome ssDNA-binding protein	-
ACE4RG_RS02380	cch1	486807..488594 (+)	1788	WP_000122621.1	cassette chromosome replicative helicase	-
ACE4RG_RS02385	ccrA	488828..490177 (+)	1350	WP_000815652.1	cassette chromosome recombinase CcrA	Machinery gene
ACE4RG_RS02390	ccrB	490199..491827 (+)	1629	WP_001186604.1	cassette chromosome recombinase CcrB	Machinery gene
ACE4RG_RS02395	-	492349..492699 (+)	351	WP_000849672.1	SAUGI family uracil-DNA glycosylase inhibitor	-
ACE4RG_RS02400	-	492786..493097 (+)	312	WP_000700851.1	DUF960 family protein	-
ACE4RG_RS02405	-	493112..493618 (+)	507	WP_001092189.1	DUF1643 domain-containing protein	-
ACE4RG_RS02410	-	493667..495277 (+)	1611	Protein_476	IS1182 family transposase	-
ACE4RG_RS02415	-	495268..495513 (-)	246	Protein_477	type I restriction enzyme endonuclease domain-containing protein	-
ACE4RG_RS02420	-	495500..496486 (-)	987	WP_001549960.1	beta-lactam sensor/signal transducer MecR1	-
ACE4RG_RS02425	mecA	496583..498592 (+)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52616.30 Da
• Isoelectric Point: 10.0062

>NTDB_id=1165147 ACE4RG_RS02385 WP_000815652.1 488828..490177(+) (ccrA) [Staphylococcus aureus isolate H2_22S00635-1]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHILTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQAMQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=1165147 ACE4RG_RS02385 WP_000815652.1 488828..490177(+) (ccrA) [Staphylococcus aureus isolate H2_22S00635-1]
ATGAAACAAGTTATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGTGTTCATATCTTAAGTGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGATTATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGTCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACATATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATAAATGGGCATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAGCCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-248)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q8VUV4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	97.55	100	0.976
ccrA	Staphylococcus aureus COL	74.833	100	0.748