Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG41_RS24200 Genome accession   NZ_CP010151
Coordinates   4668444..4669121 (-) Length   225 a.a.
NCBI ID   WP_000178172.1    Uniprot ID   -
Organism   Escherichia coli strain D8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4663444..4674121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG41_RS24185 (RG41_22835) chiA 4664805..4667504 (+) 2700 WP_000773163.1 bifunctional chitinase/lysozyme -
  RG41_RS24190 (RG41_22840) bfd 4667673..4667867 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RG41_RS24195 (RG41_22845) bfr 4667939..4668415 (+) 477 WP_000675504.1 bacterioferritin -
  RG41_RS24200 (RG41_22850) pilD 4668444..4669121 (-) 678 WP_000178172.1 prepilin peptidase GspO Machinery gene
  RG41_RS24205 (RG41_22855) gspM 4669121..4669582 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  RG41_RS24210 (RG41_22860) gspL 4669579..4670742 (-) 1164 WP_001304915.1 type II secretion system protein GspL -
  RG41_RS24215 (RG41_22865) gspK 4670757..4671740 (-) 984 WP_001058529.1 type II secretion system minor pseudopilin GspK -
  RG41_RS24220 (RG41_22870) gspJ 4671733..4672320 (-) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  RG41_RS24225 (RG41_22875) gspI 4672313..4672690 (-) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  RG41_RS24230 (RG41_22880) gspH 4672687..4673196 (-) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  RG41_RS27305 (RG41_22885) gspG 4673204..4673641 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.70 Da        Isoelectric Point: 7.6364

>NTDB_id=116377 RG41_RS24200 WP_000178172.1 4668444..4669121(-) (pilD) [Escherichia coli strain D8]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTAPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=116377 RG41_RS24200 WP_000178172.1 4668444..4669121(-) (pilD) [Escherichia coli strain D8]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGCGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382