Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG40_RS14320 Genome accession   NZ_CP010150
Coordinates   2769106..2769783 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain D7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2764106..2774783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG40_RS14305 (RG40_13630) chiA 2765473..2768166 (+) 2694 WP_001402584.1 bifunctional chitinase/lysozyme -
  RG40_RS14310 (RG40_13635) bfd 2768335..2768529 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RG40_RS14315 (RG40_13640) bfr 2768601..2769077 (+) 477 WP_000675504.1 bacterioferritin -
  RG40_RS14320 (RG40_13645) pilD 2769106..2769783 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  RG40_RS14325 (RG40_13650) gspM 2769783..2770244 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  RG40_RS14330 (RG40_13655) gspL 2770241..2771404 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  RG40_RS14335 (RG40_13660) gspK 2771419..2772402 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RG40_RS14340 (RG40_13665) gspJ 2772395..2772982 (-) 588 WP_001402582.1 type II secretion system minor pseudopilin GspJ -
  RG40_RS14345 (RG40_13670) gspI 2772975..2773352 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RG40_RS14350 (RG40_13675) gspH 2773349..2773858 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  RG40_RS25240 (RG40_13680) gspG 2773866..2774303 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=116265 RG40_RS14320 WP_000178161.1 2769106..2769783(-) (pilD) [Escherichia coli strain D7]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=116265 RG40_RS14320 WP_000178161.1 2769106..2769783(-) (pilD) [Escherichia coli strain D7]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378