Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   THERU_RS05910 Genome accession   NZ_CP007028
Coordinates   1102628..1103959 (-) Length   443 a.a.
NCBI ID   WP_025306358.1    Uniprot ID   W0DGC2
Organism   Thermocrinis ruber strain DSM 23557     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1097628..1108959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THERU_RS05875 (THERU_06030) - 1097903..1098223 (-) 321 WP_025306351.1 hypothetical protein -
  THERU_RS05880 (THERU_06035) - 1098288..1098869 (+) 582 WP_025306352.1 RNA ligase partner protein -
  THERU_RS05885 (THERU_06040) - 1098942..1099286 (+) 345 WP_025306353.1 iron-sulfur cluster assembly accessory protein -
  THERU_RS05890 (THERU_06045) - 1099289..1099678 (-) 390 WP_025306354.1 hypothetical protein -
  THERU_RS05895 (THERU_06050) aroA 1099672..1100964 (-) 1293 WP_025306355.1 3-phosphoshikimate 1-carboxyvinyltransferase -
  THERU_RS05900 (THERU_06055) - 1101011..1101973 (-) 963 WP_169727097.1 HAMP domain-containing sensor histidine kinase -
  THERU_RS05905 (THERU_06060) - 1101987..1102631 (-) 645 WP_025306357.1 response regulator transcription factor -
  THERU_RS05910 (THERU_06065) radA 1102628..1103959 (-) 1332 WP_025306358.1 DNA repair protein RadA Machinery gene
  THERU_RS05915 (THERU_06070) moaA 1103956..1104948 (-) 993 WP_025306359.1 GTP 3',8-cyclase MoaA -
  THERU_RS05920 (THERU_06075) - 1104959..1105795 (-) 837 WP_025306360.1 MqnA/MqnD/SBP family protein -
  THERU_RS05925 (THERU_06080) - 1105814..1107385 (-) 1572 WP_025306361.1 alkaline phosphatase D family protein -
  THERU_RS08355 (THERU_06085) - 1107390..1108154 (-) 765 WP_245565805.1 energy transducer TonB -
  THERU_RS05935 (THERU_06090) - 1108223..1108645 (-) 423 WP_038532191.1 biopolymer transporter ExbD -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 49071.09 Da        Isoelectric Point: 6.3822

>NTDB_id=116049 THERU_RS05910 WP_025306358.1 1102628..1103959(-) (radA) [Thermocrinis ruber strain DSM 23557]
MKKEKTFFVCQECGYSSTKWFGRCPSCGAWNSMVEERERVATKTLGPAMVYKPLPMWVEEDLKRLSTGFEQMDLALGGGL
VKGQVLLIAGEPGIGKSTLLLQLAEKYSKAYGKVLYVSGEESGSQIGTRAKRLKVEGENLYLLPETELEYILETIQELKP
SLLVVDSIQTIHSSQLESAPGSVAQVRECAYKLAEFCKRRDIPVFIVGQITKEGSIAGPKVLEHLVDTVLYFEGERFSFY
RVLKVVKNRFGASGEVVVFKMTERGLEEVPEPSALFLQERRASPGSVVFPHTEGNKPVLLEVQALVIDALYTTPQRRTQG
YDINRLALILAVLEKEAKIFTRDKDVFVNVVGGMQIVEPAGDLAVALAIASSVKGKELGDVVVFGELGLGGEVRAVHFPE
MRLKEGKRFGFKRAMVPKGNILEMDDMEIYAVSHIKEALELLL

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=116049 THERU_RS05910 WP_025306358.1 1102628..1103959(-) (radA) [Thermocrinis ruber strain DSM 23557]
ATGAAAAAAGAAAAAACATTCTTTGTCTGTCAGGAGTGCGGATACTCTTCCACCAAGTGGTTTGGAAGGTGTCCTTCTTG
CGGTGCTTGGAACAGTATGGTGGAAGAGAGGGAAAGGGTTGCCACCAAGACCCTTGGTCCTGCCATGGTCTACAAGCCCT
TACCCATGTGGGTGGAGGAGGACTTAAAAAGGCTATCTACTGGCTTTGAACAGATGGACTTAGCCTTGGGCGGAGGGCTT
GTAAAGGGACAGGTTTTACTGATCGCAGGGGAGCCGGGCATAGGAAAATCTACCCTGCTTTTGCAGTTGGCGGAAAAGTA
CTCAAAGGCTTACGGAAAGGTTCTGTATGTATCAGGGGAAGAGTCAGGCAGTCAGATAGGAACGAGGGCAAAAAGGCTAA
AAGTGGAAGGGGAAAACCTCTACCTTTTGCCCGAGACTGAGCTTGAGTATATTTTGGAAACCATCCAAGAACTAAAGCCA
AGCCTTTTGGTAGTGGATTCCATACAGACCATCCATTCTTCCCAGTTGGAATCTGCACCCGGTTCAGTGGCACAGGTTAG
GGAGTGTGCGTACAAGCTGGCAGAGTTCTGCAAAAGAAGGGACATCCCAGTGTTCATAGTAGGACAGATCACCAAGGAGG
GAAGCATTGCAGGTCCAAAGGTTCTGGAACATCTGGTGGATACAGTCCTTTATTTTGAAGGAGAAAGGTTCAGCTTTTAC
CGAGTTTTAAAGGTGGTTAAGAATCGCTTTGGTGCAAGTGGGGAGGTGGTGGTTTTTAAGATGACGGAAAGGGGATTGGA
GGAGGTGCCCGAGCCATCAGCCCTATTTTTGCAAGAGAGAAGGGCAAGCCCGGGCAGTGTGGTATTTCCTCACACGGAGG
GCAACAAGCCAGTGCTTTTGGAGGTGCAAGCCTTAGTCATAGACGCCCTGTACACAACACCTCAGAGAAGGACCCAGGGC
TATGACATAAACCGCCTTGCCCTTATCCTTGCGGTCCTTGAGAAGGAGGCTAAAATATTCACAAGGGATAAAGATGTCTT
TGTGAACGTGGTGGGGGGTATGCAGATCGTGGAACCTGCGGGGGACCTGGCGGTAGCCTTAGCCATAGCATCCTCTGTGA
AAGGAAAGGAGCTGGGGGATGTGGTGGTCTTTGGAGAGCTGGGCTTGGGTGGAGAGGTCAGGGCGGTGCATTTTCCAGAG
ATGAGACTAAAAGAGGGCAAACGCTTTGGCTTTAAAAGGGCAATGGTTCCAAAGGGCAACATCCTTGAGATGGATGATAT
GGAAATATACGCAGTTTCCCACATAAAAGAAGCCCTTGAGCTTTTGCTATGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-214)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0DGC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

47.368

100

0.488
  radA Streptococcus pneumoniae Rx1

45.333

100

0.46
  radA Streptococcus pneumoniae D39

45.333

100

0.46
  radA Streptococcus pneumoniae R6

45.333

100

0.46
  radA Streptococcus pneumoniae TIGR4

45.333

100

0.46
  radA Streptococcus mitis NCTC 12261

45.333

100

0.46
  radA Streptococcus mitis SK321

45.333

100

0.46

Multiple sequence alignment