Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   QOS64_RS13675 Genome accession   NZ_OX460973
Coordinates   2760916..2761683 (-) Length   255 a.a.
NCBI ID   WP_012118014.1    Uniprot ID   A7Z6U2
Organism   Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2755916..2766683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOS64_RS13655 - 2756118..2756930 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  QOS64_RS13660 ccpN 2756956..2757594 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  QOS64_RS13665 glyS 2757695..2759734 (-) 2040 WP_059368104.1 glycine--tRNA ligase subunit beta -
  QOS64_RS13670 glyQ 2759727..2760614 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  QOS64_RS13675 recO 2760916..2761683 (-) 768 WP_012118014.1 DNA repair protein RecO Machinery gene
  QOS64_RS13680 - 2761720..2761863 (-) 144 WP_007408286.1 YqzL family protein -
  QOS64_RS13685 era 2762019..2762924 (-) 906 WP_007408285.1 GTPase Era -
  QOS64_RS13690 - 2762905..2763315 (-) 411 WP_059368102.1 cytidine deaminase -
  QOS64_RS13695 - 2763439..2763813 (-) 375 WP_003152974.1 diacylglycerol kinase family protein -
  QOS64_RS13700 ybeY 2763794..2764267 (-) 474 WP_136396496.1 rRNA maturation RNase YbeY -
  QOS64_RS13705 - 2764268..2766400 (-) 2133 WP_007408284.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29310.95 Da        Isoelectric Point: 8.5625

>NTDB_id=1159457 QOS64_RS13675 WP_012118014.1 2760916..2761683(-) (recO) [Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPMSPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1159457 QOS64_RS13675 WP_012118014.1 2760916..2761683(-) (recO) [Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACAGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATGTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAATGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z6U2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

88.627

100

0.886

Multiple sequence alignment