Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   QOS64_RS13170 Genome accession   NZ_OX460973
Coordinates   2675637..2676437 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2670637..2681437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOS64_RS13140 - 2671174..2671806 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  QOS64_RS13145 - 2672103..2672240 (+) 138 WP_014470646.1 hypothetical protein -
  QOS64_RS13150 - 2672334..2673452 (+) 1119 WP_015240192.1 NADH-dependent flavin oxidoreductase -
  QOS64_RS13155 - 2673580..2673792 (+) 213 WP_007408362.1 spore germination protein -
  QOS64_RS13160 - 2673918..2674130 (+) 213 WP_003153181.1 spore germination protein -
  QOS64_RS13165 alr 2674307..2675488 (+) 1182 WP_015240193.1 alanine racemase -
  QOS64_RS13170 spo0A 2675637..2676437 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  QOS64_RS13175 spoIVB 2676718..2677998 (-) 1281 WP_076424958.1 SpoIVB peptidase -
  QOS64_RS13180 recN 2678162..2679892 (-) 1731 WP_136396491.1 DNA repair protein RecN Machinery gene
  QOS64_RS13185 argR 2679924..2680373 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  QOS64_RS13190 - 2680470..2681315 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=1159448 QOS64_RS13170 WP_003153177.1 2675637..2676437(-) (spo0A) [Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1159448 QOS64_RS13170 WP_003153177.1 2675637..2676437(-) (spo0A) [Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGTATTATCCGCAGCCAGCCGGAACCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTAGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment