Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RG34_RS09155 Genome accession   NZ_CP010134
Coordinates   1752861..1753301 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain D1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1747861..1758301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG34_RS09130 (RG34_08505) aroP 1748859..1750232 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  RG34_RS09135 (RG34_08510) ampE 1750275..1751129 (-) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  RG34_RS09140 (RG34_08515) ampD 1751126..1751677 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG34_RS09145 (RG34_08520) nadC 1751765..1752658 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG34_RS09155 (RG34_08530) pilA 1752861..1753301 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  RG34_RS09160 (RG34_08535) pilB 1753311..1754696 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  RG34_RS09165 (RG34_08540) hofC 1754686..1755888 (+) 1203 WP_000157236.1 protein transport protein HofC -
  RG34_RS09170 (RG34_08545) guaC 1755923..1756966 (-) 1044 WP_001217338.1 GMP reductase -
  RG34_RS29045 - 1757122..1757166 (-) 45 WP_120795372.1 protein YacM -
  RG34_RS09175 (RG34_08550) coaE 1757191..1757811 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=115832 RG34_RS09155 WP_000360895.1 1752861..1753301(+) (pilA) [Escherichia coli strain D1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=115832 RG34_RS09155 WP_000360895.1 1752861..1753301(+) (pilA) [Escherichia coli strain D1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363