Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP56_RS02320 Genome accession   NZ_OX419652
Coordinates   418133..418582 (-) Length   149 a.a.
NCBI ID   WP_070548532.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6131     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 413133..423582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP56_RS02280 - 413456..413653 (+) 198 WP_122060560.1 hypothetical protein -
  KJP56_RS02285 (NRS6131_02315) yckC 413677..414126 (+) 450 WP_032724907.1 RDD family protein -
  KJP56_RS02290 (NRS6131_02320) yckD 414208..414540 (+) 333 WP_032724908.1 YckD family protein -
  KJP56_RS02295 (NRS6131_02325) - 414668..415090 (+) 423 WP_213419590.1 MarR family transcriptional regulator -
  KJP56_RS02300 (NRS6131_02330) - 415078..415419 (+) 342 WP_213419533.1 DUF3147 family protein -
  KJP56_RS02305 (NRS6131_02335) - 415438..416166 (+) 729 WP_021481826.1 AAA family ATPase -
  KJP56_RS02310 (NRS6131_02340) bglC 416238..417671 (+) 1434 WP_123373936.1 6-phospho-beta-glucosidase -
  KJP56_RS02315 (NRS6131_02345) nin/comJ 417708..418106 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  KJP56_RS02320 (NRS6131_02350) nucA/comI 418133..418582 (-) 450 WP_070548532.1 DNA-entry nuclease Machinery gene
  KJP56_RS02325 (NRS6131_02355) tlpC 418750..420471 (-) 1722 WP_213419534.1 methyl-accepting chemotaxis protein -
  KJP56_RS02330 (NRS6131_02360) hxlB 420582..421139 (-) 558 WP_041850679.1 6-phospho-3-hexuloisomerase -
  KJP56_RS02335 (NRS6131_02365) hxlA 421145..421777 (-) 633 WP_021481819.1 3-hexulose-6-phosphate synthase -
  KJP56_RS02340 (NRS6131_02370) hxlR 422009..422371 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.35 Da        Isoelectric Point: 4.4397

>NTDB_id=1158036 KJP56_RS02320 WP_070548532.1 418133..418582(-) (nucA/comI) [Bacillus subtilis isolate NRS6131]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLQDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1158036 KJP56_RS02320 WP_070548532.1 418133..418582(-) (nucA/comI) [Bacillus subtilis isolate NRS6131]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTACAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment