Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP45_RS12795 Genome accession   NZ_OX419578
Coordinates   2486964..2487374 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis isolate NRS6128     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2481964..2492374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP45_RS12765 - 2482051..2482191 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KJP45_RS12770 (NRS6128_12680) yqeF 2482497..2483228 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  KJP45_RS12775 (NRS6128_12685) cwlH 2483480..2484232 (-) 753 WP_087961324.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  KJP45_RS12780 (NRS6128_12690) yqeD 2484419..2485045 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  KJP45_RS12785 (NRS6128_12695) gnd 2485064..2485957 (-) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KJP45_RS12790 (NRS6128_12700) yqeB 2486209..2486931 (+) 723 WP_010886572.1 hypothetical protein -
  KJP45_RS12795 (NRS6128_12705) nucA/comI 2486964..2487374 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KJP45_RS12800 (NRS6128_12710) sigK 2487570..2488298 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  KJP45_RS12805 (NRS6128_12715) - 2488870..2489550 (-) 681 WP_015714344.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
  KJP45_RS12810 (NRS6128_12720) - 2489681..2491024 (+) 1344 WP_038429355.1 MATE family efflux transporter -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1157839 KJP45_RS12795 WP_009967785.1 2486964..2487374(-) (nucA/comI) [Bacillus subtilis isolate NRS6128]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1157839 KJP45_RS12795 WP_009967785.1 2486964..2487374(-) (nucA/comI) [Bacillus subtilis isolate NRS6128]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment