Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP45_RS01970 Genome accession   NZ_OX419578
Coordinates   367021..367470 (-) Length   149 a.a.
NCBI ID   WP_129092925.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6128     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 362021..372470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP45_RS01930 (NRS6128_01915) - 362349..362545 (+) 197 Protein_336 hypothetical protein -
  KJP45_RS01935 (NRS6128_01920) yckC 362564..363013 (+) 450 WP_087614278.1 RDD family protein -
  KJP45_RS01940 (NRS6128_01925) yckD 363095..363427 (+) 333 WP_003246247.1 YckD family protein -
  KJP45_RS01945 (NRS6128_01930) - 363555..363977 (+) 423 WP_198297576.1 MarR family transcriptional regulator -
  KJP45_RS01950 (NRS6128_01935) - 363965..364306 (+) 342 WP_087614281.1 DUF3147 family protein -
  KJP45_RS01955 (NRS6128_01940) - 364325..365053 (+) 729 WP_087614282.1 AAA family ATPase -
  KJP45_RS01960 (NRS6128_01945) bglC 365126..366559 (+) 1434 WP_087614284.1 6-phospho-beta-glucosidase -
  KJP45_RS01965 (NRS6128_01950) nin/comJ 366596..366994 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  KJP45_RS01970 (NRS6128_01955) nucA/comI 367021..367470 (-) 450 WP_129092925.1 DNA-entry nuclease Machinery gene
  KJP45_RS01975 (NRS6128_01960) tlpC 367638..369359 (-) 1722 WP_087614285.1 methyl-accepting chemotaxis protein TlpC -
  KJP45_RS01980 (NRS6128_01965) hxlB 369470..370027 (-) 558 WP_086343442.1 6-phospho-3-hexuloisomerase -
  KJP45_RS01985 (NRS6128_01970) hxlA 370033..370665 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  KJP45_RS01990 (NRS6128_01975) hxlR 370897..371259 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=1157793 KJP45_RS01970 WP_129092925.1 367021..367470(-) (nucA/comI) [Bacillus subtilis isolate NRS6128]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETITFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1157793 KJP45_RS01970 WP_129092925.1 367021..367470(-) (nucA/comI) [Bacillus subtilis isolate NRS6128]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCACCTTTCCTTCTGACCGTT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment