Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KJP47_RS00585 Genome accession   NZ_OX419577
Coordinates   107331..108707 (+) Length   458 a.a.
NCBI ID   WP_213383246.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6120     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 102331..113707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP47_RS00565 (NRS6120_00615) ctsR 102684..103148 (+) 465 WP_041337889.1 transcriptional regulator CtsR -
  KJP47_RS00570 mcsA 103162..103719 (+) 558 WP_213383248.1 UvrB/UvrC motif-containing protein -
  KJP47_RS00575 (NRS6120_00625) mcsB 103719..104810 (+) 1092 WP_003235007.1 protein arginine kinase -
  KJP47_RS00580 (NRS6120_00630) clpC 104807..107239 (+) 2433 WP_213383247.1 ATP-dependent Clp protease ATP-binding subunit Regulator
  KJP47_RS00585 (NRS6120_00635) radA 107331..108707 (+) 1377 WP_213383246.1 DNA repair protein RadA Machinery gene
  KJP47_RS00590 (NRS6120_00640) disA 108711..109793 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  KJP47_RS00595 (NRS6120_00645) yacL 109909..111009 (+) 1101 WP_043858286.1 PIN/TRAM domain-containing protein -
  KJP47_RS00600 (NRS6120_00650) ispD 111024..111722 (+) 699 WP_213383245.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KJP47_RS00605 (NRS6120_00655) ispF 111715..112191 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49468.96 Da        Isoelectric Point: 8.2399

>NTDB_id=1157715 KJP47_RS00585 WP_213383246.1 107331..108707(+) (radA) [Bacillus subtilis isolate NRS6120]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPTFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=1157715 KJP47_RS00585 WP_213383246.1 107331..108707(+) (radA) [Bacillus subtilis isolate NRS6120]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCCGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAACGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAGTGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGTTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGTTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTAGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.563

100

0.996
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624

Multiple sequence alignment