Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP51_RS01975 Genome accession   NZ_OX419576
Coordinates   370776..371225 (-) Length   149 a.a.
NCBI ID   WP_033883649.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6105     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365776..376225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP51_RS01940 (NRS6105_01935) yckA 366045..366725 (-) 681 WP_213380016.1 amino acid ABC transporter permease -
  KJP51_RS01945 (NRS6105_01940) yckB 366735..367598 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  KJP51_RS01950 - 367760..367957 (+) 198 Protein_340 hypothetical protein -
  KJP51_RS01955 (NRS6105_01950) - 367994..368314 (+) 321 Protein_341 RDD family protein -
  KJP51_RS01960 (NRS6105_01955) yckD 368396..368728 (+) 333 WP_213380012.1 YckD family protein -
  KJP51_RS01965 (NRS6105_01960) bglC 368881..370314 (+) 1434 WP_213380010.1 family 1 glycosylhydrolase -
  KJP51_RS01970 (NRS6105_01965) nin/comJ 370351..370749 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  KJP51_RS01975 (NRS6105_01970) nucA/comI 370776..371225 (-) 450 WP_033883649.1 DNA-entry nuclease Machinery gene
  KJP51_RS01980 (NRS6105_01975) tlpC 371393..373114 (-) 1722 WP_213380008.1 methyl-accepting chemotaxis protein -
  KJP51_RS01985 (NRS6105_01980) hxlB 373225..373782 (-) 558 WP_024572182.1 6-phospho-3-hexuloisomerase -
  KJP51_RS01990 (NRS6105_01985) hxlA 373788..374420 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  KJP51_RS01995 (NRS6105_01990) hxlR 374653..375015 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16454.42 Da        Isoelectric Point: 4.5282

>NTDB_id=1157634 KJP51_RS01975 WP_033883649.1 370776..371225(-) (nucA/comI) [Bacillus subtilis isolate NRS6105]
MNITTDIIKTILLVIVIIAAAAIGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1157634 KJP51_RS01975 WP_033883649.1 370776..371225(-) (nucA/comI) [Bacillus subtilis isolate NRS6105]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTATCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment