Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   KJP57_RS20930 Genome accession   NZ_OX419573
Coordinates   3963859..3965004 (+) Length   381 a.a.
NCBI ID   WP_017696253.1    Uniprot ID   A0A0C3JF17
Organism   Bacillus subtilis isolate NRS6085     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3958859..3970004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP57_RS20910 (NRS6085_20825) albF 3958875..3960155 (+) 1281 WP_213407856.1 pitrilysin family protein -
  KJP57_RS20915 (NRS6085_20830) albG 3960152..3960853 (+) 702 WP_003243391.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  KJP57_RS20920 (NRS6085_20835) ywhL 3960859..3962235 (-) 1377 WP_128738345.1 YncE family protein -
  KJP57_RS20925 (NRS6085_20840) ywhK 3962274..3963629 (-) 1356 WP_015250986.1 YncE family protein -
  KJP57_RS22905 - 3963626..3963856 (-) 231 WP_024572000.1 hypothetical protein -
  KJP57_RS20930 (NRS6085_20845) rapF 3963859..3965004 (+) 1146 WP_017696253.1 tetratricopeptide repeat protein Regulator
  KJP57_RS20935 (NRS6085_20850) - 3964988..3965107 (+) 120 WP_014665774.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  KJP57_RS20940 (NRS6085_20855) - 3965206..3965668 (+) 463 Protein_4071 YbaK/EbsC family protein -
  KJP57_RS20945 (NRS6085_20860) speB 3965708..3966580 (-) 873 WP_003227545.1 agmatinase -
  KJP57_RS20950 (NRS6085_20865) speE 3966641..3967471 (-) 831 WP_003227543.1 spermidine synthase -
  KJP57_RS20955 (NRS6085_20870) pbpG 3967673..3969748 (+) 2076 WP_015384942.1 transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45593.24 Da        Isoelectric Point: 5.0684

>NTDB_id=1157537 KJP57_RS20930 WP_017696253.1 3963859..3965004(+) (rapF) [Bacillus subtilis isolate NRS6085]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDRREYVLAIQFFKKAESKLVFVKDKIDKAEFFFKMSEVYYYMKQTYVS
MDYARQAFEIYSEQTTYDIRMIQCQSLFGANFLDLKQYEKAIAHFQKAFTLAKTEQQPQLMGRTLYNIALCKQNQNSFES
AIEYVKKSIQVFEGSKMLPSLPQAFFLITQIYFKLGKIDNACEYHKKGKEYSAAAGDTVYLTEFEFLESLYLSGPDEERI
EKCFVFLESKMMYADIEDFALDVAKYYHQQENYKKASHYFLKVEEARQHIQGGVNLYEMEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1157537 KJP57_RS20930 WP_017696253.1 3963859..3965004(+) (rapF) [Bacillus subtilis isolate NRS6085]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTATGAGCTGGATCGCCGTGAATATGTATTAGCCATTCAATTCTTTAAAAAAGCTGAGAGCAAGCTGGTTTTTG
TCAAAGATAAAATCGATAAAGCTGAATTCTTTTTCAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTCTCC
ATGGACTATGCACGTCAAGCATTCGAAATATACAGTGAACAGACTACATATGATATCAGAATGATTCAATGCCAGTCCTT
ATTCGGAGCCAACTTTTTGGATTTAAAACAATACGAAAAAGCAATTGCCCATTTTCAAAAAGCCTTTACACTTGCAAAAA
CAGAGCAGCAGCCCCAATTAATGGGAAGAACACTTTATAACATAGCACTCTGCAAGCAAAACCAAAACAGTTTTGAATCA
GCGATAGAGTATGTAAAAAAATCTATTCAAGTATTTGAAGGATCTAAAATGCTTCCTTCATTGCCACAAGCTTTTTTCCT
AATTACACAAATTTATTTTAAATTAGGTAAGATTGATAACGCCTGTGAATATCATAAAAAAGGAAAAGAGTATTCTGCAG
CTGCGGGAGATACCGTGTATCTAACGGAGTTTGAATTTTTGGAATCTTTATACTTGTCTGGACCGGATGAGGAACGGATA
GAAAAATGTTTTGTTTTTCTTGAAAGTAAAATGATGTATGCAGATATTGAGGACTTCGCACTTGATGTTGCTAAATATTA
TCATCAACAGGAAAATTACAAAAAAGCATCTCATTACTTTTTAAAGGTTGAAGAAGCAAGACAGCACATTCAAGGGGGGG
TGAATTTATATGAAATGGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Tetratricopeptide repeat (TPR_8)

(263-290)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C3JF17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

80.052

100

0.801
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57

Multiple sequence alignment