Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   KJP57_RS14300 Genome accession   NZ_OX419573
Coordinates   2704817..2705227 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis isolate NRS6085     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 2699817..2710227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP57_RS14270 - 2699904..2700044 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KJP57_RS14275 (NRS6085_14285) yqeF 2700350..2701081 (-) 732 WP_128738026.1 SGNH/GDSL hydrolase family protein -
  KJP57_RS14280 (NRS6085_14290) cwlH 2701333..2702085 (-) 753 WP_213407811.1 N-acetylmuramoyl-L-alanine amidase -
  KJP57_RS14285 (NRS6085_14295) yqeD 2702272..2702898 (+) 627 WP_128738028.1 TVP38/TMEM64 family protein -
  KJP57_RS14290 (NRS6085_14300) gnd 2702918..2703811 (-) 894 WP_046160611.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KJP57_RS14295 (NRS6085_14305) yqeB 2704063..2704785 (+) 723 WP_128738029.1 DUF308 domain-containing protein -
  KJP57_RS14300 (NRS6085_14310) nucA/comI 2704817..2705227 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KJP57_RS14305 (NRS6085_14315) sigK 2705423..2706151 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  KJP57_RS14310 - 2706151..2706267 (+) 117 WP_041850087.1 hypothetical protein -
  KJP57_RS14315 (NRS6085_14320) - 2706233..2706430 (-) 198 Protein_2775 recombinase family protein -
  KJP57_RS14320 - 2706388..2706566 (-) 179 Protein_2776 hypothetical protein -
  KJP57_RS14325 (NRS6085_14325) fumC 2706691..2708079 (-) 1389 WP_128738030.1 class II fumarate hydratase -
  KJP57_RS14330 (NRS6085_14330) - 2708246..2709136 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1157514 KJP57_RS14300 WP_009967785.1 2704817..2705227(-) (nucA/comI) [Bacillus subtilis isolate NRS6085]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1157514 KJP57_RS14300 WP_009967785.1 2704817..2705227(-) (nucA/comI) [Bacillus subtilis isolate NRS6085]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529


Multiple sequence alignment