Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP57_RS02365 Genome accession   NZ_OX419573
Coordinates   422348..422797 (-) Length   149 a.a.
NCBI ID   WP_213407910.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6085     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 417348..427797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP57_RS02325 - 417670..417866 (+) 197 Protein_415 hypothetical protein -
  KJP57_RS02330 yckC 417885..418340 (+) 456 Protein_416 RDD family protein -
  KJP57_RS02335 (NRS6085_02355) yckD 418422..418754 (+) 333 WP_015252849.1 YckD family protein -
  KJP57_RS02340 (NRS6085_02360) - 418882..419304 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  KJP57_RS02345 (NRS6085_02365) - 419292..419633 (+) 342 WP_032724911.1 DUF3147 family protein -
  KJP57_RS02350 (NRS6085_02370) - 419652..420380 (+) 729 WP_213407913.1 AAA family ATPase -
  KJP57_RS02355 (NRS6085_02375) bglC 420453..421886 (+) 1434 WP_213407912.1 family 1 glycosylhydrolase -
  KJP57_RS02360 (NRS6085_02380) nin/comJ 421923..422321 (-) 399 WP_213407911.1 competence protein ComJ Regulator
  KJP57_RS02365 (NRS6085_02385) nucA/comI 422348..422797 (-) 450 WP_213407910.1 DNA-entry nuclease Machinery gene
  KJP57_RS02370 (NRS6085_02390) tlpC 422965..424686 (-) 1722 WP_213407909.1 methyl-accepting chemotaxis protein -
  KJP57_RS02375 (NRS6085_02395) hxlB 424797..425354 (-) 558 WP_213407908.1 6-phospho-3-hexuloisomerase -
  KJP57_RS02380 (NRS6085_02400) hxlA 425360..425992 (-) 633 WP_131227103.1 3-hexulose-6-phosphate synthase -
  KJP57_RS02385 (NRS6085_02405) hxlR 426225..426587 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16426.37 Da        Isoelectric Point: 4.5282

>NTDB_id=1157468 KJP57_RS02365 WP_213407910.1 422348..422797(-) (nucA/comI) [Bacillus subtilis isolate NRS6085]
MNITTDIVKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1157468 KJP57_RS02365 WP_213407910.1 422348..422797(-) (nucA/comI) [Bacillus subtilis isolate NRS6085]
GTGAACATCACGACGGACATCGTAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAAGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment