Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP64_RS12830 Genome accession   NZ_OX419565
Coordinates   2493378..2493788 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis isolate NRS6145     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2488378..2498788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP64_RS12800 - 2488465..2488605 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KJP64_RS12805 (NRS6145_12680) yqeF 2488911..2489642 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  KJP64_RS12810 (NRS6145_12685) cwlH 2489894..2490646 (-) 753 WP_046381197.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  KJP64_RS12815 (NRS6145_12690) yqeD 2490833..2491459 (+) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  KJP64_RS12820 (NRS6145_12695) gnd 2491478..2492371 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KJP64_RS12825 (NRS6145_12700) yqeB 2492623..2493345 (+) 723 WP_213379493.1 DUF308 domain-containing protein -
  KJP64_RS12830 (NRS6145_12705) nucA/comI 2493378..2493788 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KJP64_RS12835 (NRS6145_12710) - 2493984..2494331 (+) 348 Protein_2480 sigma-70 family RNA polymerase sigma factor -
  KJP64_RS12840 (NRS6145_12715) spoIVCA 2494362..2495822 (-) 1461 WP_250620044.1 site-specific DNA recombinase SpoIVCA -
  KJP64_RS12845 - 2495780..2495958 (-) 179 Protein_2482 hypothetical protein -
  KJP64_RS12850 (NRS6145_12725) - 2496871..2497083 (+) 213 WP_029726695.1 spore germination protein -
  KJP64_RS12855 - 2497451..2497544 (+) 94 Protein_2484 spore germination protein -
  KJP64_RS12860 (NRS6145_12730) - 2497828..2498538 (-) 711 WP_029726694.1 DUF421 domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1157177 KJP64_RS12830 WP_009967785.1 2493378..2493788(-) (nucA/comI) [Bacillus subtilis isolate NRS6145]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1157177 KJP64_RS12830 WP_009967785.1 2493378..2493788(-) (nucA/comI) [Bacillus subtilis isolate NRS6145]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment