Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP48_RS13915 Genome accession   NZ_OX419564
Coordinates   2642650..2643060 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis isolate NRS6127     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2637650..2648060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP48_RS13885 - 2637772..2637912 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KJP48_RS13890 (NRS6127_13815) yqeF 2638218..2638949 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  KJP48_RS13895 (NRS6127_13820) cwlH 2639201..2639953 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  KJP48_RS13900 (NRS6127_13825) yqeD 2640140..2640766 (+) 627 WP_029726699.1 TVP38/TMEM64 family protein -
  KJP48_RS13905 (NRS6127_13835) gnd 2640785..2641653 (-) 869 Protein_2692 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KJP48_RS13910 (NRS6127_13840) yqeB 2641895..2642617 (+) 723 WP_010886572.1 hypothetical protein -
  KJP48_RS13915 (NRS6127_13845) nucA/comI 2642650..2643060 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KJP48_RS13920 (NRS6127_13850) - 2643256..2643603 (+) 348 Protein_2695 sigma-70 family RNA polymerase sigma factor -
  KJP48_RS13925 (NRS6127_13855) spoIVCA 2643634..2645094 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  KJP48_RS13930 - 2645052..2645230 (-) 179 Protein_2697 hypothetical protein -
  KJP48_RS13935 (NRS6127_13860) arsC 2645585..2646004 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  KJP48_RS13940 (NRS6127_13865) acr3 2646016..2647056 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  KJP48_RS13945 (NRS6127_13870) yqcK 2647079..2647519 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  KJP48_RS13950 (NRS6127_13875) arsR 2647580..2647897 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1157093 KJP48_RS13915 WP_009967785.1 2642650..2643060(-) (nucA/comI) [Bacillus subtilis isolate NRS6127]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1157093 KJP48_RS13915 WP_009967785.1 2642650..2643060(-) (nucA/comI) [Bacillus subtilis isolate NRS6127]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment