Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   KJP75_RS12995 Genome accession   NZ_OX419560
Coordinates   2516231..2516641 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis isolate NRS6132     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 2511231..2521641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP75_RS12965 - 2511343..2511483 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KJP75_RS12970 (NRS6132_12890) yqeF 2511789..2512520 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  KJP75_RS12975 (NRS6132_12895) cwlH 2512772..2513524 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  KJP75_RS12980 (NRS6132_12900) yqeD 2513711..2514337 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  KJP75_RS12985 (NRS6132_12910) gnd 2514356..2515224 (-) 869 Protein_2508 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KJP75_RS12990 (NRS6132_12915) yqeB 2515476..2516198 (+) 723 WP_010886572.1 hypothetical protein -
  KJP75_RS12995 (NRS6132_12920) nucA/comI 2516231..2516641 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KJP75_RS13000 (NRS6132_12925) - 2516837..2517184 (+) 348 Protein_2511 sigma-70 family RNA polymerase sigma factor -
  KJP75_RS13005 (NRS6132_12930) spoIVCA 2517215..2518675 (-) 1461 WP_250620044.1 site-specific DNA recombinase SpoIVCA -
  KJP75_RS13010 - 2518633..2518811 (-) 179 Protein_2513 hypothetical protein -
  KJP75_RS13015 (NRS6132_12940) - 2519724..2519936 (+) 213 WP_029726695.1 spore germination protein -
  KJP75_RS13020 - 2520304..2520397 (+) 94 Protein_2515 spore germination protein -
  KJP75_RS13025 (NRS6132_12945) - 2520681..2521391 (-) 711 WP_029726694.1 DUF421 domain-containing protein -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1156928 KJP75_RS12995 WP_009967785.1 2516231..2516641(-) (nucA/comI) [Bacillus subtilis isolate NRS6132]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1156928 KJP75_RS12995 WP_009967785.1 2516231..2516641(-) (nucA/comI) [Bacillus subtilis isolate NRS6132]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529


Multiple sequence alignment