Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   KJP68_RS12985 Genome accession   NZ_OX419559
Coordinates   2514640..2515050 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis isolate NRS6099     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 2509640..2520050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP68_RS12955 - 2509752..2509892 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KJP68_RS12960 (NRS6099_12890) yqeF 2510198..2510929 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  KJP68_RS12965 (NRS6099_12895) cwlH 2511181..2511933 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  KJP68_RS12970 (NRS6099_12900) yqeD 2512120..2512746 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  KJP68_RS12975 (NRS6099_12910) gnd 2512765..2513633 (-) 869 Protein_2506 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KJP68_RS12980 (NRS6099_12915) yqeB 2513885..2514607 (+) 723 WP_010886572.1 hypothetical protein -
  KJP68_RS12985 (NRS6099_12920) nucA/comI 2514640..2515050 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KJP68_RS12990 (NRS6099_12925) - 2515246..2515593 (+) 348 Protein_2509 sigma-70 family RNA polymerase sigma factor -
  KJP68_RS12995 (NRS6099_12930) spoIVCA 2515624..2517084 (-) 1461 WP_250620044.1 site-specific DNA recombinase SpoIVCA -
  KJP68_RS13000 - 2517042..2517220 (-) 179 Protein_2511 hypothetical protein -
  KJP68_RS13005 (NRS6099_12940) - 2518133..2518345 (+) 213 WP_029726695.1 spore germination protein -
  KJP68_RS13010 - 2518713..2518806 (+) 94 Protein_2513 spore germination protein -
  KJP68_RS13015 (NRS6099_12945) - 2519090..2519800 (-) 711 WP_029726694.1 DUF421 domain-containing protein -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1156849 KJP68_RS12985 WP_009967785.1 2514640..2515050(-) (nucA/comI) [Bacillus subtilis isolate NRS6099]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1156849 KJP68_RS12985 WP_009967785.1 2514640..2515050(-) (nucA/comI) [Bacillus subtilis isolate NRS6099]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529


Multiple sequence alignment