Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP65_RS13255 Genome accession   NZ_OX419555
Coordinates   2524785..2525195 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis isolate NRS6181     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2519785..2530195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP65_RS13225 - 2519897..2520037 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KJP65_RS13230 (NRS6181_13185) yqeF 2520343..2521074 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  KJP65_RS13235 (NRS6181_13190) cwlH 2521326..2522078 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  KJP65_RS13240 (NRS6181_13195) yqeD 2522265..2522891 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  KJP65_RS13245 (NRS6181_13205) gnd 2522910..2523778 (-) 869 Protein_2560 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KJP65_RS13250 (NRS6181_13210) yqeB 2524030..2524752 (+) 723 WP_010886572.1 hypothetical protein -
  KJP65_RS13255 (NRS6181_13215) nucA/comI 2524785..2525195 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KJP65_RS13260 (NRS6181_13220) - 2525391..2525738 (+) 348 Protein_2563 sigma-70 family RNA polymerase sigma factor -
  KJP65_RS13265 (NRS6181_13225) spoIVCA 2525769..2527229 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  KJP65_RS13270 - 2527187..2527365 (-) 179 Protein_2565 hypothetical protein -
  KJP65_RS13275 (NRS6181_13230) arsC 2527720..2528139 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  KJP65_RS13280 (NRS6181_13235) acr3 2528151..2529191 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  KJP65_RS13285 (NRS6181_13240) yqcK 2529214..2529654 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  KJP65_RS13290 (NRS6181_13245) arsR 2529715..2530032 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1156691 KJP65_RS13255 WP_009967785.1 2524785..2525195(-) (nucA/comI) [Bacillus subtilis isolate NRS6181]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1156691 KJP65_RS13255 WP_009967785.1 2524785..2525195(-) (nucA/comI) [Bacillus subtilis isolate NRS6181]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment