Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   KJP77_RS20275 Genome accession   NZ_OX419554
Coordinates   3858424..3859569 (+) Length   381 a.a.
NCBI ID   WP_015714890.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6160     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3853424..3864569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP77_RS20255 (NRS6160_20000) albF 3853440..3854720 (+) 1281 WP_003227558.1 pitrilysin family protein -
  KJP77_RS20260 (NRS6160_20005) albG 3854717..3855418 (+) 702 WP_003227556.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  KJP77_RS20265 (NRS6160_20010) ywhL 3855424..3856800 (-) 1377 WP_015714888.1 YncE family protein -
  KJP77_RS20270 (NRS6160_20015) ywhK 3856839..3858194 (-) 1356 WP_015250986.1 YncE family protein -
  KJP77_RS22155 - 3858191..3858421 (-) 231 WP_015714889.1 hypothetical protein -
  KJP77_RS20275 (NRS6160_20020) rapF 3858424..3859569 (+) 1146 WP_015714890.1 response regulator aspartate phosphatase RapF Regulator
  KJP77_RS20280 (NRS6160_20025) phrF 3859553..3859672 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  KJP77_RS20285 (NRS6160_20035) - 3859929..3860408 (+) 480 WP_015714892.1 hypothetical protein -
  KJP77_RS20290 (NRS6160_20040) - 3860557..3861345 (+) 789 WP_015714893.1 membrane protein -
  KJP77_RS20295 (NRS6160_20045) - 3861332..3862006 (+) 675 WP_015714894.1 hypothetical protein -
  KJP77_RS20300 (NRS6160_20050) - 3862016..3862813 (+) 798 WP_231573542.1 hypothetical protein -
  KJP77_RS20305 (NRS6160_20055) - 3862816..3863463 (+) 648 WP_015714896.1 ABC transporter ATP-binding protein -
  KJP77_RS20310 (NRS6160_20060) - 3863456..3864016 (+) 561 WP_015714897.1 hypothetical protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45571.01 Da        Isoelectric Point: 4.9848

>NTDB_id=1156633 KJP77_RS20275 WP_015714890.1 3858424..3859569(+) (rapF) [Bacillus subtilis isolate NRS6160]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLNLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1156633 KJP77_RS20275 WP_015714890.1 3858424..3859569(+) (rapF) [Bacillus subtilis isolate NRS6160]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTAATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCGTCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(223-290)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

98.688

100

0.987
  rapC Bacillus subtilis subsp. subtilis str. 168

57.368

99.738

0.572

Multiple sequence alignment