Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP77_RS02330 Genome accession   NZ_OX419554
Coordinates   419494..419943 (-) Length   149 a.a.
NCBI ID   WP_157678489.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6160     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 414494..424943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP77_RS02295 (NRS6160_02295) yckA 414769..415449 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  KJP77_RS02300 (NRS6160_02300) yckB 415459..416322 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  KJP77_RS02305 - 416484..416676 (+) 193 Protein_411 hypothetical protein -
  KJP77_RS02310 - 416712..417032 (+) 321 Protein_412 RDD family protein -
  KJP77_RS02315 (NRS6160_02305) yckD 417114..417446 (+) 333 WP_015252849.1 YckD family protein -
  KJP77_RS02320 (NRS6160_02310) bglC 417599..419032 (+) 1434 WP_032722901.1 6-phospho-beta-glucosidase -
  KJP77_RS02325 (NRS6160_02315) nin/comJ 419069..419467 (-) 399 WP_032722902.1 DNA-entry nuclease inhibitor Regulator
  KJP77_RS02330 (NRS6160_02320) nucA/comI 419494..419943 (-) 450 WP_157678489.1 DNA-entry nuclease Machinery gene
  KJP77_RS02335 (NRS6160_02325) tlpC 420111..421832 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  KJP77_RS02340 (NRS6160_02330) hxlB 421943..422500 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  KJP77_RS02345 (NRS6160_02335) hxlA 422506..423138 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  KJP77_RS02350 (NRS6160_02340) hxlR 423371..423733 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16458.43 Da        Isoelectric Point: 4.5282

>NTDB_id=1156566 KJP77_RS02330 WP_157678489.1 419494..419943(-) (nucA/comI) [Bacillus subtilis isolate NRS6160]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1156566 KJP77_RS02330 WP_157678489.1 419494..419943(-) (nucA/comI) [Bacillus subtilis isolate NRS6160]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

99.329

100

0.993

Multiple sequence alignment