Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KJP78_RS01975 Genome accession   NZ_OX419553
Coordinates   370439..370888 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis isolate NRS6183     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365439..375888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP78_RS01940 (NRS6183_01915) yckA 365593..366273 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  KJP78_RS01945 (NRS6183_01920) yckB 366283..367146 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  KJP78_RS01950 - 367308..367505 (+) 198 WP_119122841.1 hypothetical protein -
  KJP78_RS01955 (NRS6183_01935) yckC 367524..367978 (+) 455 Protein_341 RDD family protein -
  KJP78_RS01960 (NRS6183_01940) yckD 368060..368392 (+) 333 WP_015252849.1 YckD family protein -
  KJP78_RS01965 (NRS6183_01945) bglC 368544..369977 (+) 1434 WP_029727076.1 6-phospho-beta-glucosidase -
  KJP78_RS01970 (NRS6183_01950) nin/comJ 370014..370412 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  KJP78_RS01975 (NRS6183_01955) nucA/comI 370439..370888 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  KJP78_RS01980 (NRS6183_01960) tlpC 371056..372777 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  KJP78_RS01985 (NRS6183_01965) hxlB 372888..373445 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  KJP78_RS01990 (NRS6183_01970) hxlA 373451..374083 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  KJP78_RS01995 (NRS6183_01975) hxlR 374316..374678 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=1156485 KJP78_RS01975 WP_003234608.1 370439..370888(-) (nucA/comI) [Bacillus subtilis isolate NRS6183]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1156485 KJP78_RS01975 WP_003234608.1 370439..370888(-) (nucA/comI) [Bacillus subtilis isolate NRS6183]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment