Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QWI75_RS09420 Genome accession   NZ_OX365700
Coordinates   1995483..1996850 (+) Length   455 a.a.
NCBI ID   WP_289268444.1    Uniprot ID   A0AA86MZ09
Organism   Nitrospira tepida strain DNF     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1990483..2001850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWI75_RS09380 (DNFV4_01946) - 1990930..1991376 (-) 447 WP_289268436.1 hypothetical protein -
  QWI75_RS09385 (DNFV4_01947) - 1991439..1992248 (-) 810 WP_289268437.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  QWI75_RS09390 (DNFV4_01948) - 1992436..1992819 (+) 384 WP_289268438.1 cyclophilin-like fold protein -
  QWI75_RS09395 (DNFV4_01949) - 1992866..1993147 (+) 282 WP_289268439.1 DUF507 family protein -
  QWI75_RS09400 (DNFV4_01950) - 1993162..1993440 (+) 279 WP_289268440.1 DUF507 family protein -
  QWI75_RS09405 (DNFV4_01951) - 1993490..1993963 (+) 474 WP_289268441.1 tetratricopeptide repeat protein -
  QWI75_RS09410 (DNFV4_01952) bcp 1994018..1994482 (+) 465 WP_289268442.1 thioredoxin-dependent thiol peroxidase -
  QWI75_RS09415 (DNFV4_01953) - 1994525..1995382 (+) 858 WP_289268443.1 hypothetical protein -
  QWI75_RS09420 (DNFV4_01954) radA 1995483..1996850 (+) 1368 WP_289268444.1 DNA repair protein RadA Machinery gene
  QWI75_RS09425 (DNFV4_01955) - 1996852..1997733 (+) 882 WP_289268445.1 hypothetical protein -
  QWI75_RS09430 (DNFV4_01956) tsaB 1997750..1998529 (+) 780 WP_289268446.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  QWI75_RS09435 (DNFV4_01957) rimI 1999016..1999537 (+) 522 WP_289268447.1 ribosomal protein S18-alanine N-acetyltransferase -
  QWI75_RS09440 (DNFV4_01958) - 1999597..1999842 (+) 246 WP_289268448.1 DUF465 domain-containing protein -
  QWI75_RS09445 (DNFV4_01959) tatC 1999911..2000693 (+) 783 WP_289268449.1 twin-arginine translocase subunit TatC -
  QWI75_RS09450 (DNFV4_01960) - 2000708..2001799 (+) 1092 WP_289268450.1 hypothetical protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49060.51 Da        Isoelectric Point: 5.2518

>NTDB_id=1155795 QWI75_RS09420 WP_289268444.1 1995483..1996850(+) (radA) [Nitrospira tepida strain DNF]
MRAKTTFHCQACGYQAARWLGRCPDCGQWNSFKEIREAPASRGRPQALTDQVAQPTALGEIELAEEPRLPTGIGELDRVL
GGGVVPGSVVLIGGDPGIGKTTLLLQALPKLAGPKERVLYVSGEESPRQIKMRANRLNVGTDSLFILSETSLQEMLNVVR
EFQPAALVVDSIQTVYSDQLTSAPGTVSQVQEVAAQLMWLAKRGGLPVFIIGHVTKEGAIAGPRLLEHIVDTVLYFEGDR
GHAYRILRAVKNRFGSTNEIGVFEMKESGLEEVSNPSELFLAERPEHSTGSVVVSSLEGTRPILCEVQALVSSTSFSMPK
RMANGVEVNRLSLLLAVMEKRLGMHVAGQDVYVNVVGGITIDEPAIDLGLVAAVTSSLREVPIDHRTVMMGEVGLGGEVR
AVTQAELRIKEAAKLGFKRCLLPERNLAKLDNLGSFEVELVGVEEIGEALEAALV

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1155795 QWI75_RS09420 WP_289268444.1 1995483..1996850(+) (radA) [Nitrospira tepida strain DNF]
ATGCGGGCCAAAACTACTTTTCACTGCCAGGCCTGCGGGTATCAGGCGGCGCGGTGGCTTGGACGGTGCCCGGACTGCGG
ACAGTGGAATAGTTTCAAGGAGATCCGCGAAGCTCCCGCCTCCCGCGGCCGTCCCCAGGCCCTTACCGATCAGGTGGCAC
AGCCCACGGCCCTTGGCGAAATCGAACTGGCGGAGGAACCGCGTCTGCCGACCGGGATCGGCGAATTGGATCGGGTCCTG
GGCGGCGGCGTCGTGCCAGGCTCGGTCGTGTTGATCGGCGGCGATCCCGGCATCGGCAAGACGACCCTGCTGTTACAGGC
CCTCCCCAAACTGGCCGGGCCGAAAGAACGGGTGCTCTACGTGTCGGGAGAGGAGTCTCCCCGGCAGATCAAAATGCGCG
CGAACCGATTGAACGTCGGGACCGATTCGCTGTTTATCCTCTCCGAAACATCCCTCCAGGAAATGCTGAACGTCGTCCGC
GAGTTCCAGCCGGCGGCGCTGGTCGTGGACTCGATCCAAACCGTCTATTCCGATCAGTTGACCTCCGCGCCCGGCACCGT
GAGCCAGGTCCAGGAAGTGGCGGCGCAGTTGATGTGGCTGGCCAAGCGCGGCGGCCTGCCCGTGTTCATCATCGGGCACG
TGACGAAGGAAGGGGCCATTGCCGGGCCGCGGCTGTTGGAACATATCGTGGACACGGTGCTGTACTTCGAGGGGGATCGG
GGGCACGCCTACCGCATTCTTCGCGCCGTCAAGAACCGCTTCGGCTCGACGAACGAGATCGGCGTGTTTGAGATGAAGGA
GAGCGGCCTCGAAGAGGTCAGCAATCCGTCGGAGCTGTTTCTGGCGGAACGGCCGGAGCACAGTACCGGGTCTGTGGTCG
TGTCCAGCCTCGAAGGGACCCGTCCGATCCTCTGCGAAGTCCAAGCATTGGTTTCCTCGACCAGCTTCAGCATGCCCAAG
CGGATGGCGAACGGGGTGGAGGTCAATCGGTTGTCGCTGCTGCTGGCCGTCATGGAGAAGCGACTCGGGATGCATGTGGC
CGGTCAGGATGTGTACGTGAACGTGGTGGGAGGAATCACGATCGATGAGCCGGCCATCGATCTCGGGCTGGTCGCCGCCG
TCACGTCGAGCTTGCGCGAGGTTCCGATCGACCATCGGACCGTCATGATGGGGGAGGTGGGGTTGGGAGGTGAAGTGCGC
GCCGTCACCCAGGCCGAGCTGCGGATCAAGGAGGCGGCCAAGCTGGGATTCAAACGGTGCCTCTTGCCGGAACGGAATTT
GGCCAAGCTGGACAATCTCGGCAGCTTCGAAGTGGAATTGGTGGGAGTTGAAGAGATCGGAGAGGCGCTTGAAGCGGCGT
TGGTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(349-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

56.14

100

0.563
  radA Streptococcus mitis SK321

50.773

99.56

0.505
  radA Streptococcus mitis NCTC 12261

50.773

99.56

0.505
  radA Streptococcus pneumoniae Rx1

50.773

99.56

0.505
  radA Streptococcus pneumoniae D39

50.773

99.56

0.505
  radA Streptococcus pneumoniae R6

50.773

99.56

0.505
  radA Streptococcus pneumoniae TIGR4

50.773

99.56

0.505

Multiple sequence alignment