Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QML74_RS00665 Genome accession   NZ_OX346404
Coordinates   127290..128660 (+) Length   456 a.a.
NCBI ID   WP_016250427.1    Uniprot ID   S1RPR1
Organism   Enterococcus cecorum isolate CIRMBP-1281     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 122290..133660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QML74_RS00645 (CIRMBP1281_00130) - 123642..124889 (-) 1248 WP_047341293.1 glutamate-5-semialdehyde dehydrogenase -
  QML74_RS00650 (CIRMBP1281_00131) proB 124905..125708 (-) 804 WP_235426847.1 glutamate 5-kinase -
  QML74_RS00655 (CIRMBP1281_00132) - 126024..126533 (-) 510 WP_016250429.1 hypothetical protein -
  QML74_RS00660 (CIRMBP1281_00133) - 126756..127277 (+) 522 WP_026209954.1 hypothetical protein -
  QML74_RS00665 (CIRMBP1281_00134) radA 127290..128660 (+) 1371 WP_016250427.1 DNA repair protein RadA Machinery gene
  QML74_RS00670 (CIRMBP1281_00135) - 128860..129966 (+) 1107 WP_016250426.1 PIN/TRAM domain-containing protein -
  QML74_RS00675 (CIRMBP1281_00136) rfbC 130006..130563 (+) 558 WP_016250425.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  QML74_RS00680 (CIRMBP1281_00137) - 130857..131126 (+) 270 WP_235426474.1 hypothetical protein -
  QML74_RS00685 - 131195..131323 (+) 129 WP_018345333.1 hypothetical protein -
  QML74_RS00690 (CIRMBP1281_00138) - 131348..131671 (+) 324 WP_016250423.1 GNAT family N-acetyltransferase -
  QML74_RS00695 (CIRMBP1281_00139) gltX 131766..133223 (+) 1458 WP_016250422.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49914.75 Da        Isoelectric Point: 7.3668

>NTDB_id=1155020 QML74_RS00665 WP_016250427.1 127290..128660(+) (radA) [Enterococcus cecorum isolate CIRMBP-1281]
MAKKAKTQFECQHCGYISPRYLGKCPNCNSWNSMMEVKIENTTDVRQAVSFTGIKSQPTKITEVVPKKTPCVQTEIGELN
RVLGGGVVPGSLILLGGDPGIGKSTLLLQVSEQLTKAHGKVLYVSGEESAEQIKMRAERLGVLHESFYVYAENDMEAIRQ
AIEQLSPDYVIIDSIQTMIQPSISSVAGSVSQVRETTAELMRIAKSNQIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GERQQSFRILRAVKNRFGSTNEIGIFEMHEDGLHEVINPSQVFLEERLQGATGSAIVVTMEGTRPLLVEIQALITPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGMLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQATDCFIGEIGLTG
EIRRVNAIEQRIKEVQKLGFKRVFVPKNNLANLEIPAGIEVIGVSTLAETMKKVFG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1155020 QML74_RS00665 WP_016250427.1 127290..128660(+) (radA) [Enterococcus cecorum isolate CIRMBP-1281]
GTGGCAAAAAAAGCAAAAACACAATTTGAGTGTCAACATTGTGGCTATATTTCCCCACGTTATTTAGGGAAATGTCCGAA
TTGTAATAGTTGGAACTCGATGATGGAAGTCAAAATCGAAAATACCACCGATGTCCGTCAAGCTGTTTCGTTTACCGGAA
TCAAGAGCCAACCAACCAAAATTACCGAAGTGGTGCCGAAAAAAACACCGTGCGTCCAAACAGAAATTGGTGAGTTGAAT
CGCGTGCTAGGTGGCGGCGTGGTGCCAGGTTCCTTGATTTTACTAGGAGGCGATCCGGGTATTGGTAAGTCGACACTGTT
ATTACAAGTATCCGAGCAGCTAACCAAAGCCCACGGGAAAGTCTTGTACGTCTCAGGTGAAGAAAGTGCCGAGCAAATCA
AGATGCGTGCGGAACGTCTAGGGGTCTTGCATGAGTCCTTTTATGTCTATGCGGAAAATGATATGGAGGCCATTCGTCAA
GCCATCGAACAACTTTCGCCTGATTATGTGATTATTGATTCGATTCAAACCATGATACAGCCAAGCATTTCTTCTGTGGC
GGGAAGTGTCAGTCAGGTTCGCGAAACCACCGCTGAGTTGATGCGCATTGCTAAGAGTAATCAGATTGCCATTTTTATCG
TTGGTCATGTGACAAAAGAAGGGGCACTAGCAGGTCCTAGAATGTTAGAACACATGGTTGATACCGTGTTATATTTTGAA
GGAGAACGCCAACAGTCCTTTAGAATTTTACGTGCGGTGAAAAATCGTTTCGGTTCGACCAATGAAATCGGCATTTTCGA
GATGCATGAAGATGGGTTGCATGAAGTCATCAATCCTTCGCAAGTCTTTTTAGAAGAACGTCTGCAAGGAGCGACTGGTT
CGGCAATCGTGGTGACGATGGAAGGAACGCGCCCATTATTAGTGGAGATTCAAGCGTTGATTACACCCACCATGTTTGGA
AATGCCAAGCGTACCACGACAGGTCTAGACTTTAATCGGGTATCTTTAATTATGGCGGTCTTGGAAAAGCGCGCTGGAAT
GTTATTGCAAAATCAAGATGCCTATTTAAAAGCAGCCGGTGGTGTGAAGCTAAACGAGCCAGCAATCGATTTAGCTATTG
CGGTGAGTATTGCCTCTAGTTACAAGGAAAAAGGCACCCAGGCAACGGATTGTTTTATCGGTGAAATTGGCTTGACCGGT
GAAATCAGACGCGTCAATGCCATCGAACAACGCATCAAAGAAGTGCAAAAGTTAGGCTTTAAACGGGTATTTGTACCTAA
AAATAATTTAGCTAATTTAGAAATTCCAGCGGGTATTGAAGTTATCGGGGTTTCTACTTTGGCTGAAACGATGAAAAAAG
TCTTTGGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(74-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S1RPR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

70.549

99.781

0.704
  radA Streptococcus pneumoniae D39

70.549

99.781

0.704
  radA Streptococcus pneumoniae R6

70.549

99.781

0.704
  radA Streptococcus pneumoniae TIGR4

70.549

99.781

0.704
  radA Streptococcus mitis SK321

70.549

99.781

0.704
  radA Streptococcus mitis NCTC 12261

70.549

99.781

0.704
  radA Bacillus subtilis subsp. subtilis str. 168

62.637

99.781

0.625

Multiple sequence alignment