Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QML50_RS00435 Genome accession   NZ_OX346403
Coordinates   85414..86784 (+) Length   456 a.a.
NCBI ID   WP_047333966.1    Uniprot ID   A0A200I045
Organism   Enterococcus cecorum isolate CIRMBP-1283     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 80414..91784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QML50_RS00415 (CIRMBP1283_00078) - 81765..83012 (-) 1248 WP_281956594.1 glutamate-5-semialdehyde dehydrogenase -
  QML50_RS00420 (CIRMBP1283_00079) proB 83028..83831 (-) 804 WP_235426847.1 glutamate 5-kinase -
  QML50_RS11330 - 83971..84129 (-) 159 WP_345741130.1 hypothetical protein -
  QML50_RS11335 (CIRMBP1283_00080) - 84147..84416 (-) 270 WP_341482095.1 hypothetical protein -
  QML50_RS11340 (CIRMBP1283_00081) - 84367..84657 (-) 291 WP_345741131.1 hypothetical protein -
  QML50_RS00430 (CIRMBP1283_00082) - 84880..85401 (+) 522 WP_026209954.1 hypothetical protein -
  QML50_RS00435 (CIRMBP1283_00083) radA 85414..86784 (+) 1371 WP_047333966.1 DNA repair protein RadA Machinery gene
  QML50_RS00440 (CIRMBP1283_00084) - 86847..87953 (+) 1107 WP_168930943.1 PIN/TRAM domain-containing protein -
  QML50_RS00445 (CIRMBP1283_00085) rfbC 87993..88550 (+) 558 WP_047241735.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  QML50_RS00450 (CIRMBP1283_00086) - 88824..89093 (+) 270 WP_281956595.1 hypothetical protein -
  QML50_RS00455 (CIRMBP1283_00087) - 89162..89689 (+) 528 WP_281956596.1 GNAT family N-acetyltransferase -
  QML50_RS00460 (CIRMBP1283_00088) gltX 89732..91189 (+) 1458 WP_281956597.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49967.80 Da        Isoelectric Point: 7.7241

>NTDB_id=1154977 QML50_RS00435 WP_047333966.1 85414..86784(+) (radA) [Enterococcus cecorum isolate CIRMBP-1283]
MAKKAKTQFECQHCGYISPRYLGKCPNCNSWNSMMEVKIENTTDVRQAVSFTGIKSQPTKITEVVPKKTPRVQTEIGELN
RVLGGGVVPGSLILLGGDPGIGKSTLLLQVSEQLTKAHGKVLYVSGEESAEQIKMRAERLGVLHESFYVYAENDMEAIRQ
AIEQLSPDYVIIDSIQTMIQPSISSVAGSVSQVRETTAELMRIAKSNQIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GERQQSFRILRAVKNRFGSTNEIGIFEMHEDGLHEVINPSQVFLEERLQGATGSAIVVTMEGTRPLLVEIQALITPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGMLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQATDCFIGEIGLTG
EIRRVNAIEQRIKEVQKLGFKRVFVPKNNLANLEIPAGIEVIGVSTLAETMKKVFG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1154977 QML50_RS00435 WP_047333966.1 85414..86784(+) (radA) [Enterococcus cecorum isolate CIRMBP-1283]
GTGGCAAAAAAAGCAAAAACACAATTTGAGTGTCAACATTGTGGCTATATTTCCCCACGTTATTTAGGGAAATGTCCGAA
TTGTAATAGTTGGAACTCGATGATGGAAGTCAAAATCGAAAATACCACCGATGTCCGTCAAGCTGTTTCGTTTACCGGAA
TCAAGAGCCAACCAACCAAAATTACCGAAGTGGTGCCGAAAAAAACACCGCGCGTCCAAACAGAAATTGGTGAGTTGAAT
CGCGTGCTAGGTGGCGGCGTGGTTCCTGGTTCCTTGATTTTACTAGGAGGCGATCCGGGTATTGGCAAGTCGACACTGTT
ATTACAAGTATCCGAGCAGCTAACCAAAGCCCACGGGAAAGTCTTATACGTCTCAGGGGAAGAAAGTGCTGAGCAAATCA
AGATGCGTGCGGAACGTCTAGGGGTCTTGCATGAGTCCTTTTATGTCTATGCGGAAAATGATATGGAGGCCATTCGTCAA
GCCATCGAACAACTTTCGCCTGATTATGTGATTATTGATTCGATTCAAACCATGATTCAGCCAAGCATTTCTTCTGTGGC
GGGAAGTGTCAGTCAGGTCCGCGAAACCACCGCTGAATTGATGCGCATAGCCAAGAGTAATCAGATTGCCATTTTTATCG
TTGGTCATGTGACAAAAGAAGGGGCACTAGCAGGTCCTAGAATGTTAGAACACATGGTTGATACCGTGTTATATTTTGAA
GGAGAACGCCAACAGTCCTTTAGAATTTTGCGTGCGGTGAAAAATCGTTTCGGTTCGACCAATGAAATCGGCATTTTCGA
GATGCATGAAGACGGCTTGCATGAAGTCATCAATCCTTCGCAAGTCTTTTTAGAAGAACGTCTGCAAGGAGCGACTGGTT
CGGCAATCGTGGTGACGATGGAAGGAACGCGCCCATTATTAGTGGAGATTCAAGCTTTGATTACACCCACCATGTTTGGA
AATGCCAAGCGTACCACGACAGGTCTAGACTTTAATCGGGTATCTTTAATTATGGCGGTCTTGGAAAAGCGTGCCGGAAT
GTTATTGCAAAATCAAGATGCCTATTTAAAAGCTGCCGGTGGTGTGAAGCTAAACGAGCCAGCAATCGATTTAGCCATTG
CGGTGAGTATTGCCTCTAGTTACAAGGAAAAAGGCACCCAGGCAACGGATTGTTTTATCGGTGAAATCGGCTTGACTGGG
GAAATTAGACGGGTCAATGCCATCGAACAACGCATCAAAGAAGTGCAAAAGTTAGGCTTTAAACGAGTGTTTGTACCTAA
AAATAATTTAGCTAATTTAGAAATCCCAGCGGGCATTGAAGTTATCGGGGTTTCGACCTTGGCTGAAACGATGAAAAAAG
TCTTTGGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A200I045

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

70.769

99.781

0.706
  radA Streptococcus pneumoniae D39

70.769

99.781

0.706
  radA Streptococcus pneumoniae R6

70.769

99.781

0.706
  radA Streptococcus pneumoniae TIGR4

70.769

99.781

0.706
  radA Streptococcus mitis NCTC 12261

70.769

99.781

0.706
  radA Streptococcus mitis SK321

70.769

99.781

0.706
  radA Bacillus subtilis subsp. subtilis str. 168

62.857

99.781

0.627

Multiple sequence alignment