Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QML67_RS00345 Genome accession   NZ_OX346401
Coordinates   68820..70190 (+) Length   456 a.a.
NCBI ID   WP_047333966.1    Uniprot ID   A0A200I045
Organism   Enterococcus cecorum isolate CIRMBP-1246     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 63820..75190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QML67_RS00320 (CIRMBP1246_00059) - 65395..66642 (-) 1248 WP_281957430.1 glutamate-5-semialdehyde dehydrogenase -
  QML67_RS00325 (CIRMBP1246_00060) proB 66658..67461 (-) 804 WP_345741187.1 glutamate 5-kinase -
  QML67_RS00330 (CIRMBP1246_00061) - 67594..67917 (-) 324 WP_253263315.1 cell filamentation protein Fic -
  QML67_RS00335 - 68035..68259 (-) 225 WP_243192490.1 hypothetical protein -
  QML67_RS00340 (CIRMBP1246_00062) - 68286..68807 (+) 522 WP_171378711.1 dUTP pyrophosphatase -
  QML67_RS00345 (CIRMBP1246_00063) radA 68820..70190 (+) 1371 WP_047333966.1 DNA repair protein RadA Machinery gene
  QML67_RS00350 (CIRMBP1246_00064) - 70387..71493 (+) 1107 WP_168930943.1 PIN/TRAM domain-containing protein -
  QML67_RS00355 (CIRMBP1246_00065) - 71857..72099 (+) 243 WP_281957434.1 hypothetical protein -
  QML67_RS00360 (CIRMBP1246_00066) - 72168..72644 (+) 477 WP_171378706.1 GNAT family N-acetyltransferase -
  QML67_RS00365 (CIRMBP1246_00067) gltX 72739..74196 (+) 1458 WP_281957435.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49967.80 Da        Isoelectric Point: 7.7241

>NTDB_id=1154893 QML67_RS00345 WP_047333966.1 68820..70190(+) (radA) [Enterococcus cecorum isolate CIRMBP-1246]
MAKKAKTQFECQHCGYISPRYLGKCPNCNSWNSMMEVKIENTTDVRQAVSFTGIKSQPTKITEVVPKKTPRVQTEIGELN
RVLGGGVVPGSLILLGGDPGIGKSTLLLQVSEQLTKAHGKVLYVSGEESAEQIKMRAERLGVLHESFYVYAENDMEAIRQ
AIEQLSPDYVIIDSIQTMIQPSISSVAGSVSQVRETTAELMRIAKSNQIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GERQQSFRILRAVKNRFGSTNEIGIFEMHEDGLHEVINPSQVFLEERLQGATGSAIVVTMEGTRPLLVEIQALITPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGMLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQATDCFIGEIGLTG
EIRRVNAIEQRIKEVQKLGFKRVFVPKNNLANLEIPAGIEVIGVSTLAETMKKVFG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1154893 QML67_RS00345 WP_047333966.1 68820..70190(+) (radA) [Enterococcus cecorum isolate CIRMBP-1246]
GTGGCAAAAAAAGCAAAAACACAATTTGAGTGTCAACATTGTGGCTATATTTCCCCACGTTATTTAGGGAAATGTCCGAA
TTGTAATAGTTGGAACTCGATGATGGAAGTCAAAATCGAAAATACCACCGATGTCCGTCAAGCTGTTTCGTTTACCGGAA
TTAAGAGCCAACCAACCAAAATTACCGAAGTGGTGCCGAAAAAAACACCGCGCGTCCAAACAGAAATTGGTGAGTTGAAT
CGCGTGCTAGGTGGCGGCGTGGTGCCAGGTTCTTTGATTTTACTAGGAGGCGATCCGGGTATTGGTAAGTCGACACTGTT
ATTACAAGTGTCCGAGCAGCTAACCAAAGCCCATGGGAAAGTCTTGTACGTCTCAGGGGAAGAAAGTGCCGAGCAAATCA
AGATGCGTGCGGAACGTCTAGGGGTCTTGCATGAGTCCTTTTATGTCTATGCGGAAAATGATATGGAGGCCATTCGTCAA
GCCATCGAACAACTTTCGCCTGATTATGTGATTATTGATTCGATTCAAACCATGATTCAGCCAAGCATTTCTTCTGTGGC
GGGAAGTGTCAGTCAGGTCCGCGAAACCACCGCTGAGTTGATGCGCATAGCCAAGAGTAATCAGATTGCCATTTTTATCG
TTGGTCATGTGACAAAAGAAGGGGCACTAGCAGGTCCTAGAATGTTAGAACACATGGTTGATACCGTGTTATATTTTGAA
GGAGAACGCCAACAGTCCTTTAGAATTTTGCGTGCGGTGAAAAATCGTTTCGGTTCGACCAATGAAATCGGCATTTTCGA
GATGCATGAAGACGGCTTACATGAAGTCATCAATCCTTCGCAAGTCTTTTTAGAAGAACGTCTGCAAGGAGCGACGGGTT
CGGCAATCGTGGTGACGATGGAAGGAACGCGCCCATTATTAGTGGAGATTCAAGCTTTGATTACACCCACCATGTTTGGA
AATGCCAAGCGTACCACGACAGGTCTAGACTTTAATCGGGTATCTTTAATTATGGCGGTCTTGGAAAAGCGTGCCGGAAT
GTTATTGCAAAATCAAGATGCCTATTTAAAAGCTGCCGGTGGTGTGAAGCTAAACGAGCCAGCAATCGATTTAGCCATTG
CGGTGAGTATTGCCTCTAGTTACAAGGAAAAAGGCACCCAGGCAACGGATTGTTTTATCGGTGAAATTGGCTTGACCGGG
GAAATCAGACGCGTCAATGCCATCGAACAACGCATCAAAGAAGTGCAAAAGTTAGGCTTTAAACGAGTGTTTGTACCTAA
AAATAATTTAGCTAATTTAGAAATCCCAGCGGGCATTGAAGTTATCGGGGTTTCGACCTTGGCTGAAACGATGAAAAAAG
TCTTTGGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A200I045

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

70.769

99.781

0.706
  radA Streptococcus pneumoniae D39

70.769

99.781

0.706
  radA Streptococcus pneumoniae R6

70.769

99.781

0.706
  radA Streptococcus pneumoniae TIGR4

70.769

99.781

0.706
  radA Streptococcus mitis NCTC 12261

70.769

99.781

0.706
  radA Streptococcus mitis SK321

70.769

99.781

0.706
  radA Bacillus subtilis subsp. subtilis str. 168

62.857

99.781

0.627

Multiple sequence alignment