Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BAMY6614_RS05320 Genome accession   NZ_CP006960
Coordinates   1055198..1055635 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens UMAF6614     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1050198..1060635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAMY6614_RS05295 (BAMY6614_05560) - 1051218..1052354 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  BAMY6614_RS05300 (BAMY6614_05565) - 1052369..1052803 (+) 435 WP_061861834.1 RDD family protein -
  BAMY6614_RS05305 (BAMY6614_05570) - 1052920..1053198 (+) 279 WP_263612330.1 YckD family protein -
  BAMY6614_RS05310 (BAMY6614_05575) - 1053302..1054738 (+) 1437 WP_061861835.1 glycoside hydrolase family 1 protein -
  BAMY6614_RS05315 (BAMY6614_05580) nin/comJ 1054779..1055177 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  BAMY6614_RS05320 (BAMY6614_05585) nucA/comI 1055198..1055635 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BAMY6614_RS05325 (BAMY6614_05590) hxlB 1055966..1056523 (-) 558 WP_061861836.1 6-phospho-3-hexuloisomerase -
  BAMY6614_RS05330 (BAMY6614_05595) hxlA 1056520..1057155 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  BAMY6614_RS05335 (BAMY6614_05600) - 1057387..1057749 (+) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=115304 BAMY6614_RS05320 WP_003156588.1 1055198..1055635(-) (nucA/comI) [Bacillus amyloliquefaciens UMAF6614]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=115304 BAMY6614_RS05320 WP_003156588.1 1055198..1055635(-) (nucA/comI) [Bacillus amyloliquefaciens UMAF6614]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment