Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NIU15_RS12550 Genome accession   NZ_OW622535
Coordinates   2903460..2904968 (-) Length   502 a.a.
NCBI ID   WP_004195216.1    Uniprot ID   A0AAX1X7H0
Organism   Burkholderia mallei strain 34 isolate 34     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2898460..2909968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIU15_RS12525 - 2899474..2900106 (-) 633 WP_004195230.1 LON peptidase substrate-binding domain-containing protein -
  NIU15_RS12530 rapZ 2900133..2901026 (-) 894 WP_004195227.1 RNase adapter RapZ -
  NIU15_RS12535 hprK 2901112..2902080 (-) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  NIU15_RS12540 ptsN 2902209..2902718 (-) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NIU15_RS12545 raiA 2902951..2903310 (-) 360 WP_004195219.1 ribosome-associated translation inhibitor RaiA -
  NIU15_RS12550 rpoN/rpoN1 2903460..2904968 (-) 1509 WP_004195216.1 RNA polymerase factor sigma-54 Machinery gene
  NIU15_RS12555 lptB 2905137..2905913 (-) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  NIU15_RS12560 lptA 2905910..2906575 (-) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  NIU15_RS12565 lptC 2906594..2907208 (-) 615 WP_004195210.1 LPS export ABC transporter periplasmic protein LptC -
  NIU15_RS12570 - 2907214..2907753 (-) 540 WP_004200146.1 HAD family hydrolase -
  NIU15_RS12575 kdsD 2907753..2908736 (-) 984 WP_004195206.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54889.04 Da        Isoelectric Point: 4.8740

>NTDB_id=1152385 NIU15_RS12550 WP_004195216.1 2903460..2904968(-) (rpoN/rpoN1) [Burkholderia mallei strain 34 isolate 34]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=1152385 NIU15_RS12550 WP_004195216.1 2903460..2904968(-) (rpoN/rpoN1) [Burkholderia mallei strain 34 isolate 34]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGTTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAAAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.691

Multiple sequence alignment