Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NXF97_RS07145 Genome accession   NZ_OV788642
Coordinates   1562300..1563214 (-) Length   304 a.a.
NCBI ID   WP_274955560.1    Uniprot ID   -
Organism   MAG: Duodenibacillus massiliensis isolate min17_bin56     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1557300..1568214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NXF97_RS07125 rpoH 1557618..1558664 (-) 1047 WP_022372760.1 RNA polymerase sigma factor RpoH -
  NXF97_RS07130 - 1559130..1560038 (-) 909 WP_074008879.1 permease-like cell division protein FtsX -
  NXF97_RS07135 - 1560026..1560730 (-) 705 WP_274955558.1 ATP-binding cassette domain-containing protein -
  NXF97_RS07140 - 1560733..1562250 (-) 1518 WP_274955559.1 Ppx/GppA phosphatase family protein -
  NXF97_RS07145 pilA 1562300..1563214 (-) 915 WP_274955560.1 signal recognition particle-docking protein FtsY Machinery gene
  NXF97_RS07150 rsmD 1563213..1563908 (+) 696 WP_274955561.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NXF97_RS07155 coaD 1563943..1564437 (+) 495 WP_274956731.1 pantetheine-phosphate adenylyltransferase -
  NXF97_RS07160 - 1564437..1564730 (+) 294 WP_022373049.1 4Fe-4S binding protein -
  NXF97_RS07165 - 1564773..1566323 (+) 1551 WP_022373048.1 Na+/H+ antiporter NhaC family protein -
  NXF97_RS07170 lptF 1566468..1567595 (+) 1128 WP_074008875.1 LPS export ABC transporter permease LptF -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 32615.57 Da        Isoelectric Point: 5.5580

>NTDB_id=1152050 NXF97_RS07145 WP_274955560.1 1562300..1563214(-) (pilA) [MAG: Duodenibacillus massiliensis isolate min17_bin56]
MGFFSRLKQGLERTRINLAGLFSGAVIDEDFFEELETSLIAADIGVESTNLILERLRDTVKLRGLKTPEEVRLALRDEIK
RILLPAQKPLDVNRTKPFVMMMAGVNGAGKTTTIGKIAKWLAADNKTVLLAAADTFRAAAREQLAVWGERNRIDVVSQTG
GDPAAVVFDAVSAGKARGLDVVIADTAGRLPTQTNLMEELGRIRRAQGKALEGAPHEVILVVDGTNGQNALAQVKAFDAF
AQLTGLIVTKLDGTAKGGVLVAITASRGDRPLPIYFVGVGEKIDDLQPFNAEAFADALVGIERE

Nucleotide


Download         Length: 915 bp        

>NTDB_id=1152050 NXF97_RS07145 WP_274955560.1 1562300..1563214(-) (pilA) [MAG: Duodenibacillus massiliensis isolate min17_bin56]
ATGGGTTTCTTTTCTCGCTTAAAACAGGGTTTGGAGCGCACGCGCATCAATCTGGCCGGTCTTTTTTCCGGCGCCGTGAT
TGACGAAGATTTCTTTGAAGAGCTCGAAACCTCACTGATTGCCGCCGATATCGGCGTTGAAAGCACGAATCTTATCTTAG
AGCGGCTGCGCGACACGGTAAAACTCAGGGGCCTTAAAACCCCGGAAGAGGTGCGCCTTGCGCTGCGCGATGAAATTAAG
CGCATCCTCCTGCCCGCGCAAAAGCCGCTTGACGTAAACCGCACCAAGCCCTTCGTGATGATGATGGCGGGCGTTAACGG
TGCCGGCAAAACCACCACAATCGGCAAGATTGCCAAGTGGCTTGCCGCCGACAATAAGACCGTGCTTTTGGCCGCTGCCG
ATACGTTTCGGGCCGCCGCGCGCGAGCAGCTTGCGGTCTGGGGCGAGCGCAACCGCATTGACGTCGTGAGCCAGACCGGG
GGCGATCCCGCGGCCGTTGTCTTTGACGCCGTGAGTGCCGGCAAGGCCCGCGGGCTTGACGTGGTGATTGCGGACACCGC
AGGACGCCTTCCCACGCAGACAAACCTCATGGAAGAACTCGGACGCATCCGCCGCGCGCAGGGTAAAGCCCTTGAAGGCG
CGCCCCACGAGGTAATCCTCGTGGTGGACGGCACCAACGGACAAAATGCCCTGGCGCAGGTCAAAGCCTTTGACGCCTTT
GCGCAGCTCACGGGCCTTATTGTCACCAAGCTTGACGGCACCGCCAAAGGCGGCGTTCTTGTGGCAATCACCGCAAGCCG
AGGCGACAGGCCGCTTCCGATATATTTTGTCGGCGTGGGTGAAAAGATTGACGATCTGCAGCCATTTAACGCCGAGGCTT
TTGCCGATGCCTTGGTGGGTATCGAGCGCGAATAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(97-299)

SRP54-type protein, helical bundle domain (SRP54_N)

(19-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

57.895

100

0.579

Multiple sequence alignment