Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   BTBSAS_RS09850 Genome accession   NZ_LT993737
Coordinates   1990267..1992075 (-) Length   602 a.a.
NCBI ID   WP_069119611.1    Uniprot ID   -
Organism   Brochothrix thermosphacta isolate CD 337     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1985267..1997075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTBSAS_RS12935 - 1986494..1986811 (-) 318 Protein_1879 NTF2-like N-terminal transpeptidase domain-containing protein -
  BTBSAS_RS09840 (BTCD_2023) - 1987142..1987870 (-) 729 WP_029091616.1 amino acid ABC transporter ATP-binding protein -
  BTBSAS_RS13080 (BTCD_2024) - 1987851..1990013 (-) 2163 WP_069119609.1 amino acid ABC transporter substrate-binding protein/permease -
  BTBSAS_RS09850 (BTCD_2025) pepF 1990267..1992075 (-) 1809 WP_069119611.1 oligoendopeptidase F Regulator
  BTBSAS_RS09855 (BTCD_2026) - 1992152..1993264 (-) 1113 WP_069119613.1 competence protein CoiA -
  BTBSAS_RS09860 (BTCD_2027) mecA 1993385..1994065 (-) 681 WP_069119615.1 adaptor protein MecA -
  BTBSAS_RS09865 (BTCD_2028) spxA 1994324..1994731 (-) 408 WP_029091611.1 transcriptional regulator SpxA -
  BTBSAS_RS09870 (BTCD_2029) trpS 1995231..1996220 (+) 990 WP_232011805.1 tryptophan--tRNA ligase -
  BTBSAS_RS09875 (BTCD_2030) - 1996283..1996750 (-) 468 WP_029091609.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 67806.86 Da        Isoelectric Point: 4.5985

>NTDB_id=1150393 BTBSAS_RS09850 WP_069119611.1 1990267..1992075(-) (pepF) [Brochothrix thermosphacta isolate CD 337]
MAGKAITRSEVPVKDTWDLTSIFETDDAWETEFKAVQVLAEEATKFQKTLGDSADQLLAALKYSDELVVRLSQVYVYASM
KHDQDTSNTEYQALNSRAESLAAKASAALSFFEPELLDIDEAVLAAFIAESDELGVYAHELEQINKKRPHTLSAPEEALL
AKASEVLGSSQNTFGILNNADLRFPTIKDENGEDVEITHGLYGPLLENSDRRVRRDAFQNVYKTYKSLLNTFASTLTGQI
KQDNFNASVRKFSTARESAVFSNNIPESVHKTLLETVGNNVDLLHRYVSLRKQVLGLDELHVYDLYTPLVADVDELSFTY
EEAKQVTINALAPLGEEYVSILKEGFDKRWVDVVENKGKRSGAYSGGSYGTNPFILLNWHDTLDNLYTLVHEFGHSVHSY
LTRKNQPAVYGDYTIFLAEIASTTNENLLTDYLLKKYDDKKIQAYIINHFLDGFKGTVFRQTQFAEFEHLIHVADQEGNA
LTAEYLTDLYYGINKKYYGDALVHDEEIGNEWARIPHFYYNYYVYQYATGFSAASALAQHILDEGAPAVERYMTFLKAGN
SDYPIDVLKAAGVDMTTAKPIEDALKVFEARLAEFEAIIIEK

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=1150393 BTBSAS_RS09850 WP_069119611.1 1990267..1992075(-) (pepF) [Brochothrix thermosphacta isolate CD 337]
ATGGCTGGAAAAGCAATTACACGCTCTGAAGTGCCTGTAAAAGACACTTGGGACTTAACAAGTATTTTTGAAACAGATGA
CGCATGGGAAACAGAATTTAAAGCTGTCCAAGTATTAGCAGAAGAAGCAACTAAATTCCAAAAAACATTAGGTGATAGTG
CAGACCAGCTTCTTGCAGCTTTAAAATATAGTGATGAGCTTGTTGTTCGTTTATCACAAGTTTATGTTTATGCAAGTATG
AAACATGATCAAGATACTAGCAATACAGAGTATCAAGCGTTAAATAGTCGTGCAGAATCATTGGCTGCCAAAGCCTCAGC
TGCACTATCTTTCTTTGAACCGGAATTATTAGATATTGATGAAGCGGTACTTGCAGCCTTTATTGCTGAATCTGACGAAT
TAGGTGTATATGCGCATGAGTTAGAACAAATCAATAAGAAACGTCCTCATACATTGTCTGCTCCTGAGGAAGCGTTATTA
GCTAAAGCAAGCGAAGTATTAGGCTCATCACAAAATACTTTTGGTATTCTAAATAATGCAGATTTACGTTTTCCAACAAT
CAAAGATGAAAATGGTGAAGATGTAGAGATTACACACGGTCTATACGGTCCATTATTGGAAAATAGCGATCGTCGTGTGC
GTCGTGACGCTTTCCAAAATGTGTATAAAACGTATAAGTCATTGCTTAATACATTTGCCTCAACGTTGACTGGTCAAATT
AAGCAAGATAACTTCAATGCATCTGTTCGTAAGTTTAGTACTGCCAGAGAGTCGGCTGTTTTCAGCAATAATATTCCTGA
GTCAGTTCATAAAACATTGCTTGAAACGGTAGGGAATAATGTTGATTTATTACATCGTTATGTGAGTTTACGCAAGCAGG
TATTAGGTCTTGACGAATTGCATGTTTACGATCTTTACACACCGCTTGTAGCGGATGTAGATGAGCTTTCATTTACGTAT
GAAGAAGCTAAACAAGTAACAATTAATGCCTTAGCACCTTTGGGTGAAGAATATGTTTCTATCCTTAAAGAAGGTTTTGA
TAAGCGTTGGGTAGATGTGGTTGAAAATAAAGGGAAACGTAGTGGCGCATACTCTGGCGGTTCATACGGTACCAATCCAT
TTATTCTATTAAACTGGCATGATACATTAGATAATTTGTATACATTAGTTCATGAGTTTGGGCACAGTGTGCATAGTTAT
CTCACTCGAAAAAATCAACCGGCAGTTTATGGCGATTATACAATCTTTTTAGCAGAGATTGCTTCAACTACCAATGAAAA
TTTATTGACAGATTATCTTCTGAAAAAATATGATGATAAAAAAATCCAAGCCTATATTATTAACCATTTCCTTGATGGTT
TTAAAGGTACTGTTTTCCGTCAAACACAATTTGCAGAATTTGAGCACCTTATTCATGTTGCTGATCAAGAAGGTAACGCT
TTAACAGCTGAATATCTAACTGATCTCTACTATGGTATTAATAAGAAATATTATGGTGATGCACTGGTACACGATGAAGA
AATTGGTAACGAATGGGCGCGTATTCCTCATTTCTACTACAATTATTACGTGTATCAATATGCAACTGGTTTTTCTGCAG
CTTCAGCTTTGGCACAACATATTTTGGACGAAGGAGCTCCAGCAGTAGAACGTTACATGACGTTCTTAAAAGCAGGTAAC
TCGGATTATCCAATCGATGTGTTGAAAGCTGCTGGCGTTGACATGACTACTGCAAAACCAATTGAGGATGCTTTGAAAGT
TTTCGAAGCACGTCTAGCTGAATTCGAAGCAATTATTATAGAAAAATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(115-184)

Peptidase family M3 (Peptidase_M3)

(205-586)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.52

98.339

0.507

Multiple sequence alignment