Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BTBSAS_RS00430 Genome accession   NZ_LT993737
Coordinates   86661..88046 (+) Length   461 a.a.
NCBI ID   WP_029091568.1    Uniprot ID   A0A1D2KGY7
Organism   Brochothrix thermosphacta isolate CD 337     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 81661..93046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTBSAS_RS00420 (BTCD_0078) - 83464..83922 (+) 459 WP_029091570.1 CtsR family transcriptional regulator -
  BTBSAS_RS00425 (BTCD_0079) - 83953..86454 (+) 2502 WP_120474666.1 ATP-dependent Clp protease ATP-binding subunit -
  BTBSAS_RS00430 (BTCD_0080) radA 86661..88046 (+) 1386 WP_029091568.1 DNA repair protein RadA Machinery gene
  BTBSAS_RS00435 (BTCD_0081) - 88062..89144 (+) 1083 WP_029091567.1 PIN/TRAM domain-containing protein -
  BTBSAS_RS00440 (BTCD_0082) ispD 89158..89856 (+) 699 WP_069118453.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BTBSAS_RS00445 (BTCD_0083) ispF 89859..90335 (+) 477 WP_029091565.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BTBSAS_RS00450 (BTCD_0084) gltX 90371..91840 (+) 1470 WP_069118452.1 glutamate--tRNA ligase -
  BTBSAS_RS00455 (BTCD_0085) epsC 92157..92795 (+) 639 WP_029091563.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50638.30 Da        Isoelectric Point: 6.4051

>NTDB_id=1150375 BTBSAS_RS00430 WP_029091568.1 86661..88046(+) (radA) [Brochothrix thermosphacta isolate CD 337]
MAKKKASTKFVCNACGYESPKWMGRCPNCSEWNQMVEFVEAKVQKGRTSFNHTEEMESQQALPLSKITSETEQRILTDMP
ELNRVLGGGLVKGSLVLVGGDPGIGKSTLLLQVSAQLTTAKRKGLYVSGEESIKQTKLRAERLNVDGDLLYVYAQTDLEV
ISKTIQTLQPEFVVIDSIQTIYHPDVTSAAGSVTQVRECTSELMRLAKTLNIAIFIVGHVTKEGTIAGPRMLEHMVDTVL
YFEGERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVDNASEVFLEERLEGAAGSAVVTSMEGTRPVLVEIQALVSPT
VYGNARRMATGMDYNRVSLIMAVLEKREGLMLQNQDAYLKAAGGVKLDEPAIDLAIAISIASSYYDTPTAAMDCFIGEVG
LTGELRRVARIDQRVQEAAKLGFKRIIIPANNIGGWTFPAEIEVVGVSTLKDAIHKAFPNR

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1150375 BTBSAS_RS00430 WP_029091568.1 86661..88046(+) (radA) [Brochothrix thermosphacta isolate CD 337]
ATGGCAAAGAAAAAAGCGAGTACTAAATTTGTCTGCAACGCCTGCGGATATGAGTCTCCAAAATGGATGGGGCGTTGCCC
TAACTGTAGCGAATGGAACCAAATGGTCGAATTTGTTGAGGCAAAAGTCCAAAAAGGACGAACTAGTTTTAATCATACAG
AAGAAATGGAAAGTCAGCAGGCATTGCCTCTTTCTAAAATAACAAGTGAAACTGAACAACGTATCTTAACAGATATGCCA
GAATTGAATCGTGTCCTCGGCGGGGGATTAGTTAAAGGTTCATTGGTGTTGGTCGGTGGTGACCCAGGGATTGGTAAATC
AACACTATTGTTGCAAGTATCAGCACAACTCACAACAGCAAAACGCAAAGGCCTTTATGTTTCGGGAGAAGAATCAATCA
AGCAAACAAAACTACGGGCTGAACGTTTAAATGTGGATGGGGATTTATTATATGTTTATGCGCAAACCGACTTAGAAGTC
ATCTCTAAAACAATTCAGACTTTACAGCCGGAATTTGTCGTGATTGATTCGATCCAAACCATCTATCACCCTGATGTGAC
AAGTGCAGCAGGGAGCGTCACACAAGTAAGAGAGTGTACGTCAGAATTGATGCGTCTTGCAAAGACGTTAAACATTGCCA
TCTTCATTGTCGGTCATGTGACAAAAGAGGGTACAATTGCAGGTCCACGTATGTTAGAACATATGGTCGACACTGTTTTA
TACTTTGAAGGTGAACGCCACCATGCCTACCGGATTTTACGCGCCGTAAAAAATAGGTTTGGATCTACCAATGAAATGGG
TATCTTTGAAATGCGCGATGTCGGTTTGGTAGAGGTCGACAACGCCTCAGAAGTATTTTTAGAAGAGCGTTTAGAAGGAG
CTGCTGGTTCTGCAGTTGTTACCTCAATGGAGGGGACACGTCCTGTTCTGGTAGAGATTCAAGCATTGGTGTCACCGACT
GTTTATGGAAATGCACGTCGAATGGCAACAGGAATGGATTATAATCGTGTTTCTCTAATTATGGCGGTACTTGAGAAACG
TGAAGGTTTAATGCTTCAAAATCAAGATGCCTATCTTAAAGCAGCAGGTGGTGTGAAGTTAGACGAGCCTGCAATTGATT
TAGCAATAGCAATAAGTATTGCATCGAGTTATTATGATACACCAACAGCAGCAATGGATTGCTTTATTGGTGAAGTCGGT
TTAACGGGAGAGTTACGTCGTGTTGCTCGTATTGATCAACGCGTACAAGAAGCTGCAAAACTAGGTTTCAAACGTATTAT
TATACCAGCGAACAATATCGGTGGTTGGACATTCCCTGCTGAGATTGAAGTAGTAGGTGTATCAACATTAAAAGATGCGA
TTCATAAAGCATTTCCAAATAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

AAA domain (AAA_25)

(66-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D2KGY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

67.756

99.566

0.675
  radA Streptococcus pneumoniae Rx1

64.13

99.783

0.64
  radA Streptococcus pneumoniae D39

64.13

99.783

0.64
  radA Streptococcus pneumoniae R6

64.13

99.783

0.64
  radA Streptococcus pneumoniae TIGR4

64.13

99.783

0.64
  radA Streptococcus mitis SK321

64.13

99.783

0.64
  radA Streptococcus mitis NCTC 12261

64.13

99.783

0.64

Multiple sequence alignment