Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DG357_RS20195 Genome accession   NZ_LT992502
Coordinates   4171067..4172500 (+) Length   477 a.a.
NCBI ID   WP_028014571.1    Uniprot ID   A0AAU7N964
Organism   Enterobacter bugandensis isolate EB-247     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4166067..4177500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DG357_RS20165 - 4166634..4167611 (+) 978 WP_028014566.1 calcium/sodium antiporter -
  DG357_RS20170 kdsD 4167625..4168611 (+) 987 WP_028014567.1 arabinose-5-phosphate isomerase KdsD -
  DG357_RS20175 kdsC 4168626..4169192 (+) 567 WP_025756695.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  DG357_RS20180 lptC 4169189..4169764 (+) 576 WP_028014568.1 LPS export ABC transporter periplasmic protein LptC -
  DG357_RS20185 lptA 4169733..4170287 (+) 555 WP_028014569.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  DG357_RS20190 lptB 4170294..4171019 (+) 726 WP_028014570.1 LPS export ABC transporter ATP-binding protein -
  DG357_RS20195 rpoN/rpoN1 4171067..4172500 (+) 1434 WP_028014571.1 RNA polymerase factor sigma-54 Machinery gene
  DG357_RS20200 hpf 4172523..4172810 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  DG357_RS20205 ptsN 4172893..4173384 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DG357_RS20210 rapZ 4173430..4174284 (+) 855 WP_014885397.1 RNase adapter RapZ -
  DG357_RS20215 npr 4174281..4174553 (+) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  DG357_RS20220 mtgA 4174595..4175320 (-) 726 WP_049136842.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  DG357_RS20225 elbB 4175317..4175970 (-) 654 WP_088204346.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54181.95 Da        Isoelectric Point: 4.3173

>NTDB_id=1150365 DG357_RS20195 WP_028014571.1 4171067..4172500(+) (rpoN/rpoN1) [Enterobacter bugandensis isolate EB-247]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIEIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1150365 DG357_RS20195 WP_028014571.1 4171067..4172500(+) (rpoN/rpoN1) [Enterobacter bugandensis isolate EB-247]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCGATGACGCCGCAGTTACAGCAGGCAATTCGTCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCCCTGCTGGAGCAAGCCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCACAGGACACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTATACCGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGCGCTATTGCCACGTCGATTGTCGACGCCGTTGATGACACCGGTTATTTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATGGGCGATGAAGAGATTGAGATTGAGGAGATTGAAGCGGTACTGAAACGCATTCAGCGCTTCGATCC
GGTCGGGGTGGCCGCGAAGGACCTGCGCGACTGTCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAGGCACGCTTAATCATCAGCGATCATCTGGATCTTCTGGCTAACCACGATTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTAAATCTGATTCAGTCCCTCGATCCGCGTCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTTATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCTGATAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTACGCCTCCATGTGTACCAGCGCGCGCAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAGAGCCTTGAGAGCCGGAATGATACCCTGCTTCGCGTGAGCCGCTGCAT
CGTCGAACAGCAGCAGGCCTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCCACTATTTCCCGCGTTACTACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAGGGTGGCGGCGAAGCCTCCTCAACGGCGATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTAAGTGACAGTAAGTTGACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment