Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   PL963_RS02135 Genome accession   NZ_LT962480
Coordinates   437581..439800 (-) Length   739 a.a.
NCBI ID   WP_024660643.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae isolate CFBP4215     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432581..444800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL963_RS02115 (CFBP4215_00402) hslU 432698..434035 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  PL963_RS02120 (CFBP4215_00403) hslV 434102..434632 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  PL963_RS02125 (CFBP4215_00404) - 435047..435742 (-) 696 WP_003403056.1 SPOR domain-containing protein -
  PL963_RS02130 (CFBP4215_00405) argS 435744..437483 (-) 1740 WP_104683612.1 arginine--tRNA ligase -
  PL963_RS02135 (CFBP4215_00406) priA 437581..439800 (-) 2220 WP_024660643.1 primosomal protein N' Machinery gene
  PL963_RS02140 (CFBP4215_00407) rpmE 439970..440191 (+) 222 WP_003403048.1 50S ribosomal protein L31 -
  PL963_RS02145 (CFBP4215_00408) - 440205..441008 (+) 804 WP_057457309.1 thermonuclease family protein -
  PL963_RS02150 (CFBP4215_00409) - 441190..442458 (+) 1269 WP_011266374.1 malic enzyme-like NAD(P)-binding protein -
  PL963_RS28330 - 442689..442817 (+) 129 Protein_404 IS110 family transposase -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81928.23 Da        Isoelectric Point: 8.5469

>NTDB_id=1148527 PL963_RS02135 WP_024660643.1 437581..439800(-) (priA) [Pseudomonas syringae pv. syringae isolate CFBP4215]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNRDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=1148527 PL963_RS02135 WP_024660643.1 437581..439800(-) (priA) [Pseudomonas syringae pv. syringae isolate CFBP4215]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCTGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGTCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTACCCGCCGACAAGCTCAAGCCGGCAATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCGCCCGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GCTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAGCGCTTCTGGCATGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAAGCCCTGACCACCCTTGCCCAGCATCCGCACGGAGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAGGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCACTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGATTCGACAGCTTTCATGCGTTTCTGCTGGCCGGTGTCACCGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGACGCCTGGC
TGGCGGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGTTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCACTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTGGGCTCGGCCACGCCGTCCCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTATGGCCTGTTGCGCCTGAATCAGCGCGCGGGCGGCGCACAACAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTTTTGGT
GTTCCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGCGTACCGCGGCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAACGCCTGGCGATTCTGTTTCC
GGACTACCCCGTGCTGCGTGTCGATCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGTCAGCCGTGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACCCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAACTGATCGTGCAGGTGGCAGG
CCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCATCCGTTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAAGCCTTGAGCGAGCGCCGTTCAGCGAGTCTGCCACCGTTTTCACACCTT
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCCGAGCAACT
GGTTGCGCAAATGGCGCTGGGCGGCATAGAGCTGCTGGGCCCGGTTCCGGCCCCGATGGAACGCCGCGCCGGCCGTTATC
GCGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCGCCACTGCATAAATTGCTTGCCACCTGGCTGCTCGCACTGGAGCAA
ATGCCCAGCGGTCGACAGGTCAGATGGTCGCTGGATGTGGATCCCGTGGATCTTTATTAA

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment