Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LSAJ54_RS08725 Genome accession   NZ_LT960790
Coordinates   1731531..1732895 (-) Length   454 a.a.
NCBI ID   WP_011375373.1    Uniprot ID   A0A094XXJ4
Organism   Latilactobacillus sakei strain J54     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1726531..1737895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSAJ54_RS08700 (LSAJ54_1763) - 1726736..1727269 (-) 534 WP_016265667.1 NYN domain-containing protein -
  LSAJ54_RS08705 (LSAJ54_1764) rlmB 1727273..1728160 (-) 888 WP_082267809.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LSAJ54_RS08710 (LSAJ54_1765) - 1728147..1728554 (-) 408 WP_004265086.1 Mini-ribonuclease 3 -
  LSAJ54_RS08715 (LSAJ54_1766) cysS 1728551..1729957 (-) 1407 WP_056947506.1 cysteine--tRNA ligase -
  LSAJ54_RS08720 (LSAJ54_1767) - 1730388..1731500 (-) 1113 WP_035146631.1 PIN/TRAM domain-containing protein -
  LSAJ54_RS08725 (LSAJ54_1768) radA 1731531..1732895 (-) 1365 WP_011375373.1 DNA repair protein RadA Machinery gene
  LSAJ54_RS08730 (LSAJ54_1769) - 1732968..1733507 (-) 540 WP_011375374.1 dUTP diphosphatase -
  LSAJ54_RS08735 (LSAJ54_1770) rpiA 1733703..1734386 (+) 684 WP_011375375.1 ribose-5-phosphate isomerase RpiA -
  LSAJ54_RS08740 (LSAJ54_1771) - 1734861..1736183 (+) 1323 WP_076632143.1 aminopeptidase C -
  LSAJ54_RS08745 (LSAJ54_1772) - 1736388..1737734 (+) 1347 WP_099947477.1 C1 family peptidase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49598.97 Da        Isoelectric Point: 7.3488

>NTDB_id=1148521 LSAJ54_RS08725 WP_011375373.1 1731531..1732895(-) (radA) [Latilactobacillus sakei strain J54]
MAKVKTQFVCQNCGYSSPRFLGRCPNCGAWNQMVEEREQPAAAAKSNFTISGRATEPEKISTVNIQKEPRVKTELNELNR
VLGGGVVPGSLILIGGDPGIGKSTLLLQVSGQLEKVGKILYVSGEESASQIKMRANRLGVNGDQLYLYPETDMGNIRHQI
ETLKPEYVVIDSIQTMSEPEVTSAVGSVSQVRQVTAELMRIAKTNQITIFVVGHVTKEGAIAGPKILEHMVDTVLYFEGD
THHTYRILRSVKNRFGSTNEIGIFEMREAGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVELQTLITPTLFGNA
KRTSSGLDHNRVSLIMAVLEKRASLMLSNQDAYLKATGGVKLDEPAIDLAMAVSIASSYRDKEIPPTDCFVGEIGLTGEI
RRVNRIEQRVGEAAKLGFKRIYVPKNNLQGWDPPTDIQVVGVTTIAETLKKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1148521 LSAJ54_RS08725 WP_011375373.1 1731531..1732895(-) (radA) [Latilactobacillus sakei strain J54]
GTGGCTAAAGTTAAAACGCAGTTTGTCTGCCAGAATTGTGGCTATAGTTCCCCCAGATTCTTGGGGCGCTGTCCGAATTG
TGGTGCTTGGAACCAAATGGTTGAAGAACGTGAACAACCAGCCGCAGCGGCAAAATCAAATTTCACAATTTCAGGGCGCG
CCACTGAACCTGAAAAAATTAGTACTGTTAATATCCAAAAAGAACCCCGTGTTAAAACGGAATTAAATGAATTGAATCGT
GTTTTAGGTGGCGGCGTTGTTCCGGGTTCACTGATTTTAATCGGTGGGGATCCTGGGATTGGAAAATCAACCTTGCTACT
ACAAGTTTCAGGGCAACTTGAAAAAGTGGGTAAGATCCTATACGTTTCAGGTGAAGAAAGTGCCTCACAAATTAAGATGC
GGGCGAATCGATTAGGGGTTAATGGCGATCAATTGTATCTGTATCCCGAAACGGATATGGGTAATATTCGCCACCAAATT
GAAACGCTCAAACCGGAATACGTCGTGATTGATTCGATTCAAACGATGAGTGAACCAGAAGTGACTTCCGCAGTCGGGAG
TGTTTCGCAAGTTCGCCAAGTAACCGCAGAATTGATGCGGATTGCTAAAACGAATCAAATTACCATCTTCGTGGTGGGAC
ATGTGACGAAGGAAGGGGCGATTGCCGGCCCTAAGATTTTGGAACATATGGTAGATACCGTGTTGTACTTTGAAGGTGAC
ACCCATCACACTTACCGGATTTTACGATCTGTTAAAAACCGGTTTGGTTCAACCAATGAAATCGGGATTTTCGAAATGCG
GGAAGCGGGCTTGCAAGAAGTCGCTAATCCATCCGAAATTTTCCTTGAAGAACGACTAGCCGGTGCGACTGGCTCAGCAG
TTGTGGTTTCAATGGAAGGGACAAGACCAATCTTGGTTGAGTTACAAACTTTGATTACCCCAACCTTGTTCGGGAATGCG
AAACGGACCTCTTCAGGGTTAGATCATAATCGGGTGTCATTGATTATGGCGGTTTTAGAAAAGCGGGCGAGTTTGATGCT
TTCAAACCAAGATGCTTATCTTAAAGCAACCGGTGGCGTTAAGTTAGATGAACCAGCGATTGATTTGGCGATGGCCGTTT
CAATTGCTTCCAGTTATCGCGATAAAGAAATTCCACCAACCGATTGTTTTGTCGGCGAAATTGGCTTGACGGGTGAAATC
CGGCGGGTCAACCGGATCGAACAACGCGTTGGCGAAGCGGCGAAGTTAGGTTTTAAACGGATTTATGTTCCTAAAAATAA
TTTGCAAGGTTGGGATCCACCAACTGATATTCAAGTCGTTGGGGTCACCACTATTGCAGAAACTTTAAAAAAAGTATTCA
ACTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(70-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A094XXJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

69.316

99.78

0.692
  radA Streptococcus mitis SK321

69.316

99.78

0.692
  radA Streptococcus pneumoniae Rx1

69.095

99.78

0.689
  radA Streptococcus pneumoniae D39

69.095

99.78

0.689
  radA Streptococcus pneumoniae R6

69.095

99.78

0.689
  radA Streptococcus pneumoniae TIGR4

69.095

99.78

0.689
  radA Bacillus subtilis subsp. subtilis str. 168

65.121

99.78

0.65

Multiple sequence alignment