Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MFPB19_RS05715 Genome accession   NZ_LT960784
Coordinates   1138775..1139425 (-) Length   216 a.a.
NCBI ID   WP_231953421.1    Uniprot ID   -
Organism   Latilactobacillus sakei isolate MFPB19     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1133775..1144425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFPB19_RS05690 (MFPB19_1184) rpsO 1134069..1134338 (-) 270 WP_011374766.1 30S ribosomal protein S15 -
  MFPB19_RS05695 (MFPB19_1185) rpsT 1134617..1134871 (+) 255 WP_011374767.1 30S ribosomal protein S20 -
  MFPB19_RS05700 (MFPB19_1186) holA 1134940..1135968 (-) 1029 WP_011374768.1 DNA polymerase III subunit delta -
  MFPB19_RS05705 (MFPB19_1187) comEC 1135965..1138136 (-) 2172 WP_231953420.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MFPB19_RS05710 (MFPB19_1188) - 1138229..1138717 (-) 489 WP_011374770.1 ComE operon protein 2 -
  MFPB19_RS05715 (MFPB19_1189) comEA 1138775..1139425 (-) 651 WP_231953421.1 helix-hairpin-helix domain-containing protein Machinery gene
  MFPB19_RS05720 (MFPB19_1190) - 1139556..1140602 (-) 1047 WP_011374772.1 SepM family pheromone-processing serine protease -
  MFPB19_RS05725 (MFPB19_1191) coaD 1140604..1141101 (-) 498 WP_011374773.1 pantetheine-phosphate adenylyltransferase -
  MFPB19_RS05730 (MFPB19_1192) rsmD 1141114..1141668 (-) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  MFPB19_RS05735 (MFPB19_1193) - 1141673..1141963 (-) 291 WP_076632420.1 YlbG family protein -
  MFPB19_RS05740 (MFPB19_1194) - 1141987..1143075 (-) 1089 WP_076632421.1 CAP-associated domain-containing protein -
  MFPB19_RS05745 (MFPB19_1195) - 1143086..1144273 (-) 1188 WP_011374777.1 FtsW/RodA/SpoVE family cell cycle protein -

Sequence

Protein


Download         Length: 216 a.a.        Molecular weight: 23459.59 Da        Isoelectric Point: 9.7029

>NTDB_id=1148433 MFPB19_RS05715 WP_231953421.1 1138775..1139425(-) (comEA) [Latilactobacillus sakei isolate MFPB19]
MLLGIIAGGFYWHQSQQKQKVTQNEQLLATSASKTKPATKKTHQAATKKKGGFIDIKGAVVYPGIYPITVSETRLFEVLK
NAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTQEQASTGTSANASSTSDTAKIDLNQA
DSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 651 bp        

>NTDB_id=1148433 MFPB19_RS05715 WP_231953421.1 1138775..1139425(-) (comEA) [Latilactobacillus sakei isolate MFPB19]
GTGCTATTGGGGATCATCGCGGGTGGCTTTTATTGGCATCAATCGCAACAAAAACAAAAAGTAACGCAAAATGAGCAACT
GCTAGCAACCAGTGCGTCTAAAACAAAACCCGCTACTAAGAAGACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTA
TCGATATAAAAGGCGCGGTGGTCTACCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAA
AATGCAGGTGGTCCGACTGATGCGGCTGATACCAAGCGGCTTAATTTAGCTCAAAAGTTAACCGATCAACTGATTATTTA
TATTCCGAAAAAAGGAGAGGTGGTTACTGATCAAGAACTCATTCAAGCGCCACAACAAATCGCAACGACCAGTGAGCGTA
CGCAAGAACAGGCATCAACTGGGACAAGTGCGAATGCATCGAGCACGAGTGACACTGCTAAAATCGATTTGAATCAGGCT
GATAGTACGCAGCTTCAGACCCTCAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGG
AAAATTTAAACAGATTGAAGATCTACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTT
GTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(54-105)

Helix-hairpin-helix motif (HHH_3)

(153-214)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

99.074

100

0.991
  comEA Lactococcus lactis subsp. cremoris KW2

38.009

100

0.389

Multiple sequence alignment