Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LSAJ64_RS09395 Genome accession   NZ_LT960781
Coordinates   1828675..1830039 (-) Length   454 a.a.
NCBI ID   WP_011375373.1    Uniprot ID   A0A094XXJ4
Organism   Latilactobacillus sakei strain J64     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1823675..1835039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSAJ64_RS09375 (LSAJ64_1891) rlmB 1824180..1825067 (-) 888 WP_082267747.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LSAJ64_RS09380 (LSAJ64_1892) - 1825054..1825461 (-) 408 WP_004265086.1 Mini-ribonuclease 3 -
  LSAJ64_RS09385 (LSAJ64_1893) cysS 1825458..1826864 (-) 1407 WP_101378063.1 cysteine--tRNA ligase -
  LSAJ64_RS09390 (LSAJ64_1894) - 1827532..1828644 (-) 1113 WP_101378064.1 PIN/TRAM domain-containing protein -
  LSAJ64_RS09395 (LSAJ64_1895) radA 1828675..1830039 (-) 1365 WP_011375373.1 DNA repair protein RadA Machinery gene
  LSAJ64_RS09400 (LSAJ64_1896) - 1830112..1830651 (-) 540 WP_101378065.1 hypothetical protein -
  LSAJ64_RS09405 (LSAJ64_1897) rpiA 1830847..1831533 (+) 687 WP_096584173.1 ribose-5-phosphate isomerase RpiA -
  LSAJ64_RS09410 (LSAJ64_1898) - 1831847..1833169 (+) 1323 WP_101378066.1 aminopeptidase C -
  LSAJ64_RS09415 (LSAJ64_1899) - 1833323..1834669 (+) 1347 WP_035146635.1 C1 family peptidase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49598.97 Da        Isoelectric Point: 7.3488

>NTDB_id=1148410 LSAJ64_RS09395 WP_011375373.1 1828675..1830039(-) (radA) [Latilactobacillus sakei strain J64]
MAKVKTQFVCQNCGYSSPRFLGRCPNCGAWNQMVEEREQPAAAAKSNFTISGRATEPEKISTVNIQKEPRVKTELNELNR
VLGGGVVPGSLILIGGDPGIGKSTLLLQVSGQLEKVGKILYVSGEESASQIKMRANRLGVNGDQLYLYPETDMGNIRHQI
ETLKPEYVVIDSIQTMSEPEVTSAVGSVSQVRQVTAELMRIAKTNQITIFVVGHVTKEGAIAGPKILEHMVDTVLYFEGD
THHTYRILRSVKNRFGSTNEIGIFEMREAGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVELQTLITPTLFGNA
KRTSSGLDHNRVSLIMAVLEKRASLMLSNQDAYLKATGGVKLDEPAIDLAMAVSIASSYRDKEIPPTDCFVGEIGLTGEI
RRVNRIEQRVGEAAKLGFKRIYVPKNNLQGWDPPTDIQVVGVTTIAETLKKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1148410 LSAJ64_RS09395 WP_011375373.1 1828675..1830039(-) (radA) [Latilactobacillus sakei strain J64]
GTGGCTAAAGTTAAAACGCAGTTTGTCTGCCAGAATTGTGGCTATAGTTCCCCCAGATTCTTGGGGCGCTGTCCGAATTG
TGGTGCTTGGAACCAAATGGTTGAAGAACGTGAACAACCAGCCGCAGCGGCAAAATCAAATTTCACAATTTCAGGGCGCG
CCACTGAACCTGAAAAAATTAGTACCGTTAATATCCAAAAAGAACCCCGTGTTAAAACGGAATTAAATGAATTGAATCGT
GTTTTAGGTGGCGGCGTTGTGCCGGGGTCACTGATTTTAATCGGTGGGGATCCTGGGATTGGAAAATCAACCTTATTACT
ACAAGTTTCAGGGCAACTTGAAAAAGTGGGTAAGATCCTATACGTTTCAGGTGAAGAAAGTGCCTCGCAAATTAAGATGC
GGGCGAATCGATTAGGTGTTAATGGCGATCAATTGTATCTGTATCCCGAAACGGATATGGGCAATATTCGCCACCAAATC
GAAACGCTCAAACCGGAATACGTCGTGATTGATTCGATTCAAACGATGAGTGAACCAGAAGTGACTTCCGCAGTCGGAAG
TGTTTCACAGGTTCGCCAAGTAACCGCAGAATTGATGCGGATTGCTAAAACGAATCAAATTACCATCTTCGTGGTGGGAC
ATGTGACGAAGGAAGGGGCGATTGCCGGCCCTAAGATTTTGGAACATATGGTGGATACCGTGTTGTACTTTGAAGGTGAC
ACCCATCACACTTACCGGATTTTACGATCTGTTAAAAACCGGTTTGGTTCAACCAATGAAATCGGGATTTTCGAAATGCG
GGAAGCGGGCTTGCAAGAAGTCGCTAATCCATCCGAAATTTTTCTTGAAGAACGACTGGCCGGTGCGACTGGTTCAGCAG
TTGTGGTTTCAATGGAAGGGACAAGACCAATCTTGGTTGAGTTACAAACTTTGATTACCCCAACCTTGTTCGGGAATGCG
AAACGGACCTCTTCAGGGTTAGATCATAATCGGGTGTCATTGATTATGGCGGTTTTAGAAAAGCGGGCGAGTTTGATGCT
TTCAAACCAAGATGCTTATCTTAAAGCAACCGGTGGCGTTAAGTTAGATGAACCAGCGATTGATTTGGCGATGGCCGTTT
CAATTGCTTCCAGTTATCGCGATAAAGAAATTCCACCAACCGATTGTTTTGTCGGCGAAATTGGCTTGACGGGTGAAATC
CGGCGGGTCAACCGGATCGAACAACGCGTTGGCGAAGCGGCGAAGTTAGGTTTTAAACGGATTTATGTTCCTAAAAATAA
TTTGCAAGGTTGGGATCCACCAACTGATATTCAAGTCGTTGGGGTCACCACTATTGCAGAAACTTTAAAAAAAGTATTCA
ACTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(70-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A094XXJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

69.316

99.78

0.692
  radA Streptococcus mitis SK321

69.316

99.78

0.692
  radA Streptococcus pneumoniae Rx1

69.095

99.78

0.689
  radA Streptococcus pneumoniae D39

69.095

99.78

0.689
  radA Streptococcus pneumoniae R6

69.095

99.78

0.689
  radA Streptococcus pneumoniae TIGR4

69.095

99.78

0.689
  radA Bacillus subtilis subsp. subtilis str. 168

65.121

99.78

0.65

Multiple sequence alignment